About Gene List

PF3D7_0310400 (PIESP1)

Genome location: Pf3D7_03_v3:447,468..451,552(+)

Genome classification: Core

Function and Localization

Product Description: parasite-infected erythrocyte surface protein

SignalP Peptide: MKVGIIFFCLFFFVVLG

# Transmembrane Domains: 0

EC Numbers: None

Curated GO (PlasmoDB):

Type GO Term Name
Component GO:0009986 cell surface
Component GO:0020002 host cell plasma membrane
Component GO:0005634 nucleus
Component GO:0085026 tubovesicular membrane network

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage LR class MCA mean MCA prop. zeros
Sporozoite possibly expressed N/A N/A
Ring expressed 0.16 0.92
Trophozoite expressed 1.04 0.56
Schizont expressed 0.88 0.65
Gametocyte expressed 0.38 0.78

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_0310400

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Non - Mutable in CDS

MIS: 0.192 | MFS: -2.732 | #Insertions: 0

PlasmoGEM Phenotype: N/A

RMgmDB ABS Phenotype: N/A

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: O77361

"Best" AlphaFill ligand hit: UMP (2'-deoxyuridine 5'-monophosphate, Local RMSD=0.14) with 6KPR (Global RMSD=20.42)

No associated EC numbers

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_176938

No human ortholog(s)

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type common rare doubleton singleton
synonymous 5 46 27 59
disruptive 41 108 59 155
missense 27 84 43 120

Any inclusion in genotype call

variant type common rare doubleton singleton
synonymous 10 103 44 75
disruptive 72 260 140 300
missense 42 189 101 194

PlasmoDB Total SNPs: 126

Non-coding: 28 | Synonymous: 48 | Nonsynonymous: 47 | Stop Codon: 3

Protein Information

Protein Length: 1294 | Molecular Weight (kDa): 154.089

UniProt ID(s): O77361

PDB ID(s): None

Isoelectric Point: 4.93

Protein Domain Annotations:

Source Family ID Description
InterPro IPR003378 Fringe-like
PFam PF02434 Fringe-like
Superfamily N/A N/A

Associated Publications

PMID Title Authors DOI/Link
10448855 The complete nucleotide sequence of chromosome 3 of Plasmodium falciparum. Bowman S, Lawson D, ..., Barrell BG 10.1038/22964
12368867 Sequence of Plasmodium falciparum chromosomes 1, 3-9 and 13. Hall N, Pain A, ..., Barrell BG 10.1038/nature01095
15287581 Proteomics approach reveals novel proteins on the surface of malaria-infectederythrocytes. Florens L, Liu X, ..., Wu Y https://pubmed.ncbi.nlm.nih.gov/15287581/
16267556 A protein interaction network of the malaria parasite Plasmodium falciparum. LaCount DJ, Vignali M, ..., Hughes RE 10.1038/nature04104
18551176 The malaria secretome: from algorithms to essential function in blood stageinfection. van Ooij C, Tamez P, ..., Haldar K https://pubmed.ncbi.nlm.nih.gov/18551176/
30379851 Identification of Plasmodium falciparum nuclear proteins by mass spectrometry andproposed protein annotation. Briquet S, Ourimi A, ..., Vaquero C 10.1371/journal.pone.0205596