PF3D7_0311200

Genome location: Pf3D7_03_v3:473,860..479,395(+)

Genome classification: Core

Function and Localization

Product Description: valine--tRNA ligase, putative

SignalP Peptide: MTILLTLFIIYVCLIGHLTKVYI

# Transmembrane Domains: 0

EC Numbers: 6.1.1.9 (Valine--tRNA ligase)

Curated GO (PlasmoDB):

Type	GO Term	Name
Component	GO:0020011	apicoplast
Component	GO:0005829	cytosol
Function	GO:0004832	valine-tRNA ligase activity
Process	GO:0006418	tRNA aminoacylation for protein translation
Process	GO:0006438	valyl-tRNA aminoacylation

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	expressed	N/A	N/A
Ring	possibly expressed	0.23	0.89
Trophozoite	expressed	0.73	0.60
Schizont	expressed	0.14	0.91
Gametocyte	possibly expressed	0.63	0.66

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_0311200

Resistome Missense Mutations: D658H

Resistome Compounds with Missense Mutations: Atovaquone

Resistome # Samples with Disruptive Mutations: 1 (1 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Non - Mutable in CDS

MIS: 0.12 | MFS: -2.924 | #Insertions: 0

PlasmoGEM Phenotype: Essential (Pb ortholog: PBANKA_0409200)

Relative Growth Rate: 0.08 ± 0.07
Confidence: 6.76

RMgmDB ABS Phenotype: N/A

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: O77354

"Best" AlphaFill ligand hit: No AlphaFill hits

BRENDA EC Inhibitors:

EC #	Name	EC Inhibitors
6.1.1.9	valine-tRNA ligase	KClarsenitetRNAVal(-CCA)Thr-tRNAValC76tRNAVal(-ACCA)L-Penicillamine1,10-phenanthrolineS-adenosylhomocysteinep-hydroxymercuribenzoateDL-2-amino-3-chlorobutyrateD-allo-2-Amino-3-chlorobutyratepyridoxal 5'-triphospho-5'-adenosine

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_532786

No human ortholog(s)

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	10	51	30	75
disruptive	58	168	102	233
missense	37	143	94	188

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	14	128	49	82
disruptive	112	361	176	345
missense	67	282	148	236

PlasmoDB Total SNPs: 264

Non-coding: 82 | Synonymous: 103 | Nonsynonymous: 79 | Stop Codon: 0

Protein Information

Protein Length: 1367 | Molecular Weight (kDa): 164.653

UniProt ID(s): O77354, Q689C3

PDB ID(s): None

Isoelectric Point: 9.12

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR001412	Aminoacyl-tRNA synthetase, class I, conserved site
InterPro	IPR002300	Aminoacyl-tRNA synthetase, class Ia
InterPro	IPR002303	Valine-tRNA ligase
InterPro	IPR009080	Aminoacyl-tRNA synthetase, class Ia, anticodon-binding
InterPro	IPR013155	Methionyl/Valyl/Leucyl/Isoleucyl-tRNA synthetase, anticodon-binding
PFam	PF00133	Aminoacyl-tRNA synthetase, class Ia
PFam	PF08264	Methionyl/Valyl/Leucyl/Isoleucyl-tRNA synthetase, anticodon-binding
Superfamily	SSF47323	Aminoacyl-tRNA synthetase, class Ia, anticodon-binding
Superfamily	SSF52374

PMID	Title	Authors	DOI/Link
10448855	The complete nucleotide sequence of chromosome 3 of Plasmodium falciparum.	Bowman S, Lawson D, ..., Barrell BG	10.1038/22964
12368867	Sequence of Plasmodium falciparum chromosomes 1, 3-9 and 13.	Hall N, Pain A, ..., Barrell BG	10.1038/nature01095
30212465	Integrative proteomics and bioinformatic prediction enable a high-confidenceapicoplast proteome in malaria parasites.	Boucher MJ, Ghosh S, ..., Yeh E	10.1371/journal.pbio.2005895