About Gene List

PF3D7_0311300

Genome location: Pf3D7_03_v3:479,460..485,830(-)

Genome classification: Core

Function and Localization

Product Description: phosphatidylinositol 3- and 4-kinase, putative

SignalP Peptide: N/A

# Transmembrane Domains: 0

EC Numbers: 2.7.1.137 (Phosphatidylinositol 3-kinase);2.7.1.67 (1-phosphatidylinositol 4-kinase)

Curated GO (PlasmoDB):

Type GO Term Name
Component GO:0005634 nucleus

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage LR class MCA mean MCA prop. zeros
Sporozoite expressed N/A N/A
Ring not expressed 0.08 0.96
Trophozoite expressed 0.62 0.65
Schizont expressed 1.12 0.42
Gametocyte expressed 1.01 0.48

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_0311300

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Non - Mutable in CDS

MIS: 0.134 | MFS: -2.974 | #Insertions: 0

PlasmoGEM Phenotype: Slow (Pb ortholog: PBANKA_0409300)

  • Relative Growth Rate: 0.64 ± 0.25
  • Confidence: 4.16

RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PBANKA_0409300)

Modification: Disrupted | RMgm-1882

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: O77353

"Best" AlphaFill ligand hit: ATP (adenosine-5'-triphosphate, Local RMSD=0.60) with 4WTV (Global RMSD=4.63)

BRENDA EC Inhibitors:

EC # Name EC Inhibitors
2.7.1.137 phosphatidylinositol 3-kinase BRENDA SOAP query unsuccessful
2.7.1.67 1-phosphatidylinositol 4-kinase ATPADPAMPGTPGDPCTPUTPKClNEMSDSGMPCDP...

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_101496

Most Similar Human Ortholog: G5E9Z4

TM-align score: 0.70 | RMSD: 3.29

Seq Identity: 0.23 | Length: 305 / 953

All Human Orthologs (OrthoMCL):

Gene ID Description
ENSG00000038210 phosphatidylinositol 4-kinase type 2 beta
ENSG00000155252 phosphatidylinositol 4-kinase type 2 alpha
ENSG00000249967 novel protein

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type common rare doubleton singleton
synonymous 6 34 25 59
disruptive 10 47 34 91
missense 9 42 32 85

Any inclusion in genotype call

variant type common rare doubleton singleton
synonymous 9 87 31 69
disruptive 21 137 81 184
missense 14 112 71 149

PlasmoDB Total SNPs: 408

Non-coding: 357 | Synonymous: 41 | Nonsynonymous: 9 | Stop Codon: 1

Protein Information

Protein Length: 953 | Molecular Weight (kDa): 110.559

UniProt ID(s): O77353, Q689C2, Q95V40

PDB ID(s): None

Isoelectric Point: 6.47

Protein Domain Annotations:

Source Family ID Description
InterPro IPR000403 Phosphatidylinositol 3-/4-kinase, catalytic domain
PFam PF00454 Phosphatidylinositol 3-/4-kinase, catalytic domain
Superfamily N/A N/A

Associated Publications

PMID Title Authors DOI/Link
10448855 The complete nucleotide sequence of chromosome 3 of Plasmodium falciparum. Bowman S, Lawson D, ..., Barrell BG 10.1038/22964
12368867 Sequence of Plasmodium falciparum chromosomes 1, 3-9 and 13. Hall N, Pain A, ..., Barrell BG 10.1038/nature01095
23181666 Organellar proteomics reveals hundreds of novel nuclear proteins in the malariaparasite Plasmodium falciparum. Oehring SC, Woodcroft BJ, ..., Voss TS 10.1186/gb-2012-13-11-r108