Product Description: kinase-related protein PKRP, putative
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: 2.7.11.1 (Non-specific serine/threonine protein kinase)
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0005813 | centrosome |
| Component | GO:0005737 | cytoplasm |
| Component | GO:1903561 | extracellular vesicle |
| Component | GO:0005634 | nucleus |
| Component | GO:0000922 | spindle pole |
| Function | GO:0004674 | protein serine/threonine kinase activity |
| Process | GO:0000278 | mitotic cell cycle |
| Process | GO:0006468 | protein phosphorylation |
| Process | GO:0032465 | regulation of cytokinesis |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | not expressed | N/A | N/A |
| Ring | not expressed | 0.04 | 0.98 |
| Trophozoite | possibly expressed | 0.12 | 0.94 |
| Schizont | possibly expressed | 0.39 | 0.79 |
| Gametocyte | expressed | 1.04 | 0.50 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_0311400
Resistome Missense Mutations: F100I
Resistome Compounds with Missense Mutations: WLW
Resistome # Samples with Disruptive Mutations: 1 (1 missense, 0 "interesting" missense)
Zhang Phenotype: Mutable in CDS
MIS: 1 | MFS: -2.03 | #Insertions: 4
PlasmoGEM Phenotype: Dispensable (Pb ortholog: PBANKA_0409400)
RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PBANKA_0409400)
Modification: Disrupted | RMgm-1883
More info: PhenoPlasm Link
AlphaFill Uniprot ID: O77365
"Best" AlphaFill ligand hit: BU1 (1,4-butanediol, Local RMSD=0.21) with 7XZQ (Global RMSD=5.50)
BRENDA EC Inhibitors:
| EC # | Name | EC Inhibitors |
|---|---|---|
| 2.7.11.1 | non-specific serine/threonine protein kinase | ATPKClDTTP53DRBEDTATBCAKN-93K252aCKI-7LAALSTAACS... |
No evidence of orthology to BindingDB entries
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 43 | 153 | 59 | 138 |
| disruptive | 163 | 388 | 226 | 569 |
| missense | 92 | 270 | 178 | 432 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 64 | 252 | 89 | 174 |
| disruptive | 280 | 834 | 384 | 775 |
| missense | 142 | 579 | 301 | 491 |
PlasmoDB Total SNPs: 490
Non-coding: 133 | Synonymous: 213 | Nonsynonymous: 144 | Stop Codon: 0
Protein Length: 2515 | Molecular Weight (kDa): 295.497
UniProt ID(s): O77365
PDB ID(s): None
Isoelectric Point: 9.95
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR000719 | Protein kinase domain |
| InterPro | IPR011009 | Protein kinase-like domain superfamily |
| PFam | PF00069 | Protein kinase domain |
| Superfamily | SSF56112 | Protein kinase-like domain superfamily |
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 10448855 | The complete nucleotide sequence of chromosome 3 of Plasmodium falciparum. | Bowman S, Lawson D, ..., Barrell BG | 10.1038/22964 |
| 12368867 | Sequence of Plasmodium falciparum chromosomes 1, 3-9 and 13. | Hall N, Pain A, ..., Barrell BG | 10.1038/nature01095 |
| 31390575 | Landscape of the Plasmodium Interactome Reveals Both Conserved andSpecies-Specific Functionality. | Hillier C, Pardo M, ..., Choudhary JS | 10.1016/j.celrep.2019.07.019 |
| 33608523 | 20S proteasomes secreted by the malaria parasite promote its growth. | Dekel E, Yaffe D, ..., Regev-Rudzki N | 10.1038/s41467-021-21344-8 |