Product Description: dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit OST1, putative
SignalP Peptide: MKLIYVTFIRFVFFIYVHG
# Transmembrane Domains: 1
EC Numbers: 2.4.99.18 (Dolichyl-diphosphooligosaccharide--protein glycotransferase)
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0020011 | apicoplast |
| Component | GO:0008250 | oligosaccharyltransferase complex |
| Process | GO:0018279 | protein N-linked glycosylation via asparagine |
| Process | GO:0006486 | protein glycosylation |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | not expressed | N/A | N/A |
| Ring | not expressed | 0.18 | 0.90 |
| Trophozoite | expressed | 0.24 | 0.85 |
| Schizont | not expressed | 0.04 | 0.97 |
| Gametocyte | expressed | 0.38 | 0.77 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_0311600
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Non - Mutable in CDS
MIS: 0.136 | MFS: -3.075 | #Insertions: 0
PlasmoGEM Phenotype: Essential (Pb ortholog: PBANKA_0409600)
RMgmDB ABS Phenotype: N/A
More info: PhenoPlasm Link
AlphaFill Uniprot ID: O77351
"Best" AlphaFill ligand hit: PH2 (2-amino-6-hydroxymethyl-7,8-dihydro-3h-pteridin-4-one, Local RMSD=0.61) with 6JWW (Global RMSD=16.04)
BRENDA EC Inhibitors:
| EC # | Name | EC Inhibitors |
|---|---|---|
| 2.4.99.18 | dolichyl-diphosphooligosaccharide-protein glycotransferase | EDTARNAiDigitoninoctylglucosideN-benzoyl-Asn-Leu-ThrN-octanoyl-Asn-Leu-ThrN-t-butoxycarbonyl-Asn-Leu-ThrArg-Asn-Gly-(Gly-allyl)-Ala-Val-methyl esterArg-Asn-Gly-(Gly-vinyl)-Ala-Val-methyl esterArg-Asn-Gly-(Gly-allylepoxide)-Ala-Val-methyl esterN-dinitrobenzoyl-Arg-Asn-Ala-epoxyethylglycine-Ala-ValArg-Asn-Gly-(Gly-epoxyethylglycine)-Ala-Val-methylester... |
Orthology to BindingDB Entries:
| UniProt | Protein Name | Species | Homology Source | BindingDB Ligands |
|---|---|---|---|---|
| P41543 | Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 1 | Saccharomyces cerevisiae | OrthoDB, OrthoMCL | Glycopeptide, 2Glycopeptide, 2aGlycopeptide, 3aGlycopeptide, 3b... |
Ortholog Group (OrthoMCL): OG6_102688
Most Similar Human Ortholog: P04843
TM-align score: 0.65 | RMSD: 3.20
Seq Identity: 0.17 | Length: 433 / 702
All Human Orthologs (OrthoMCL):
| Gene ID | Description |
|---|---|
| ENSG00000163902 | ribophorin I |
Protein Length: 702 | Molecular Weight (kDa): 84.02
UniProt ID(s): O77351
PDB ID(s): None
Isoelectric Point: 7.15
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR007676 | Ribophorin I |
| PFam | PF04597 | Ribophorin I |
| Superfamily | N/A | N/A |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 15 | 28 | 25 | 34 |
| disruptive | 18 | 57 | 26 | 67 |
| missense | 18 | 57 | 25 | 58 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 22 | 53 | 28 | 46 |
| disruptive | 33 | 114 | 47 | 164 |
| missense | 28 | 104 | 36 | 107 |
PlasmoDB Total SNPs: 226
Non-coding: 173 | Synonymous: 32 | Nonsynonymous: 21 | Stop Codon: 0
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 10448855 | The complete nucleotide sequence of chromosome 3 of Plasmodium falciparum. | Bowman S, Lawson D, ..., Barrell BG | 10.1038/22964 |
| 12368867 | Sequence of Plasmodium falciparum chromosomes 1, 3-9 and 13. | Hall N, Pain A, ..., Barrell BG | 10.1038/nature01095 |
| 30212465 | Integrative proteomics and bioinformatic prediction enable a high-confidenceapicoplast proteome in malaria parasites. | Boucher MJ, Ghosh S, ..., Yeh E | 10.1371/journal.pbio.2005895 |