Product Description: plasmepsin VI
SignalP Peptide: MPYFHIFLYILIFCVLVHI
# Transmembrane Domains: 1
EC Numbers: 3.4.23.- (Aspartic endopeptidases.)
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0044310 | osmiophilic body |
| Function | GO:0004190 | aspartic-type endopeptidase activity |
| Process | GO:0006508 | proteolysis |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | possibly expressed | N/A | N/A |
| Ring | possibly expressed | 0.01 | 0.99 |
| Trophozoite | possibly expressed | 0.03 | 0.99 |
| Schizont | expressed | 0.01 | 0.99 |
| Gametocyte | expressed | 1.17 | 0.55 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_0311700
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Mutable in CDS
MIS: 1 | MFS: -0.936 | #Insertions: 2
PlasmoGEM Phenotype: Slow (Pb ortholog: PBANKA_1034400)
RMgmDB ABS Phenotype: Not different from wild type (Pb ortholog: PBANKA_0409700)
Modification: Disrupted | RMgm-884
More info: PhenoPlasm Link
AlphaFill Uniprot ID: O77350
"Best" AlphaFill ligand hit: IVA (isovaleric acid, Local RMSD=0.40) with 3FV3 (Global RMSD=5.32)
BRENDA EC Inhibitors:
| EC # | Name | EC Inhibitors |
|---|---|---|
| 3.4.23.- | Ex: renin | I2YYKCNSDSIAANBSDANEDTAPI-3DMSOPCMBTLCK... |
Orthology to BindingDB Entries:
| UniProt | Protein Name | Species | Homology Source | BindingDB Ligands |
|---|---|---|---|---|
| Q8IM16 | Plasmepsin IV [1-448] | HOGENOM | phenyl-substituted sulfonamides, 6dphenyl-substituted sulfonamides, 6eCHEMBL588662US9353089, 117... | |
| O60989 | Plasmepsin IV, putative | Plasmodium vivax | HOGENOM | CHEMBL575115CHEMBL584189CHEMBL575150CHEMBL584190... |
Ortholog Group (OrthoMCL): OG6_100536
Most Similar Human Ortholog: F8W787
TM-align score: 0.83 | RMSD: 1.94
Seq Identity: 0.34 | Length: 329 / 432
All Human Orthologs (OrthoMCL):
| Gene ID | Description |
|---|---|
| ENSG00000117984 | cathepsin D |
| ENSG00000131400 | napsin A aspartic peptidase |
| ENSG00000196188 | cathepsin E |
| ENSG00000250644 | novel protein |
Protein Length: 432 | Molecular Weight (kDa): 49.433
UniProt ID(s): O77350
PDB ID(s): None
Isoelectric Point: 7.64
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR001461 | Aspartic peptidase A1 family |
| InterPro | IPR001969 | Aspartic peptidase, active site |
| InterPro | IPR021109 | Aspartic peptidase domain superfamily |
| InterPro | IPR033121 | Peptidase family A1 domain |
| InterPro | IPR034164 | Pepsin-like domain |
| PFam | PF00026 | Peptidase family A1 domain |
| Superfamily | SSF50630 | Aspartic peptidase domain superfamily |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 5 | 19 | 13 | 21 |
| disruptive | 6 | 25 | 13 | 25 |
| missense | 6 | 24 | 13 | 24 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 7 | 36 | 21 | 25 |
| disruptive | 11 | 44 | 24 | 63 |
| missense | 9 | 39 | 23 | 48 |
PlasmoDB Total SNPs: 277
Non-coding: 256 | Synonymous: 15 | Nonsynonymous: 6 | Stop Codon: 0
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 10448855 | The complete nucleotide sequence of chromosome 3 of Plasmodium falciparum. | Bowman S, Lawson D, ..., Barrell BG | 10.1038/22964 |
| 12368867 | Sequence of Plasmodium falciparum chromosomes 1, 3-9 and 13. | Hall N, Pain A, ..., Barrell BG | 10.1038/nature01095 |
| 28351685 | Transcript and protein expression analysis of proteases in the blood stages ofPlasmodium falciparum. | Weissbach T, Golzmann A, ..., Julius Ngwa C | 10.1016/j.exppara.2017.03.006 |