Product Description: E3 ubiquitin-protein ligase
SignalP Peptide: N/A
# Transmembrane Domains: 5
EC Numbers: 2.3.2.27 (RING-type E3 ubiquitin transferase);6.2.1.45 (E1 ubiquitin-activating enzyme);6.3.2.19 (Transferred entry: 2.3.2.23, 2.3.2.27 and 6.2.1.45)
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0020011 | apicoplast |
| Function | GO:0004842 | ubiquitin-protein transferase activity |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | expressed | N/A | N/A |
| Ring | possibly expressed | 0.04 | 0.98 |
| Trophozoite | expressed | 0.41 | 0.75 |
| Schizont | expressed | 0.21 | 0.86 |
| Gametocyte | expressed | 0.47 | 0.73 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_0312100
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Non - Mutable in CDS
MIS: 0.169 | MFS: -2.759 | #Insertions: 0
PlasmoGEM Phenotype: Dispensable (Pb ortholog: PBANKA_0410100)
RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PBANKA_0410100)
Modification: Disrupted | RMgm-1888
More info: PhenoPlasm Link
AlphaFill Uniprot ID: O77347
"Best" AlphaFill ligand hit: No AlphaFill hits
BRENDA EC Inhibitors:
| EC # | Name | EC Inhibitors |
|---|---|---|
| 2.3.2.27 | RING-type E3 ubiquitin transferase | EDTADTPAMLN4924RAPTA-CRAPTA-TRAPTA-EA1 |
| 6.2.1.45 | E1 ubiquitin-activating enzyme | LMO2PYR-41AtMUB3largazoletrichostatin Aginsenoside Reginsenoside Rg1[ubiquitin carrier protein Ubc4]-L-cysteine5'-[[(L-cysteinylglycylglycyl)sulfamoyl]amino]-5'-deoxyadenosine1-(3-chloro-4-fluorophenyl)-4-[(5-nitro-2-furyl)methylene]-3,5-pyrazolidinedione4[4-(5-nitro-furan-2-ylmethylene)-3,5-dioxo-pyrazolidin-1-yl]-benzoic acid ethyl ester(5R,8S)-11-ethenyl-5-methyl-8-(propan-2-yl)-10-oxa-3,17-dithia-7,14,19,20-tetraazatricyclo[14.2.1.1~2,5~]icosa-1(18),2(20),16(19)-triene-6,9,13-trione... |
| 6.3.2.19 | ubiquitin-protein ligase | No BRENDA inhibitors |
No evidence of orthology to BindingDB entries
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 11 | 45 | 25 | 45 |
| disruptive | 26 | 91 | 53 | 136 |
| missense | 16 | 60 | 49 | 105 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 20 | 80 | 38 | 53 |
| disruptive | 52 | 219 | 105 | 239 |
| missense | 28 | 154 | 81 | 147 |
PlasmoDB Total SNPs: 200
Non-coding: 104 | Synonymous: 59 | Nonsynonymous: 37 | Stop Codon: 0
Protein Length: 836 | Molecular Weight (kDa): 98.719
UniProt ID(s): O77347
PDB ID(s): None
Isoelectric Point: 6.57
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR001841 | Zinc finger, RING-type |
| PFam | PF13639 | Zinc finger, RING-type |
| Superfamily | SSF57850 | N/A |
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 10448855 | The complete nucleotide sequence of chromosome 3 of Plasmodium falciparum. | Bowman S, Lawson D, ..., Barrell BG | 10.1038/22964 |
| 12368867 | Sequence of Plasmodium falciparum chromosomes 1, 3-9 and 13. | Hall N, Pain A, ..., Barrell BG | 10.1038/nature01095 |
| 23785288 | An apicoplast localized ubiquitylation system is required for the import ofnuclear-encoded plastid proteins. | Agrawal S, Chung DW, ..., Le Roch KG | 10.1371/journal.ppat.1003426 |
| 30212465 | Integrative proteomics and bioinformatic prediction enable a high-confidenceapicoplast proteome in malaria parasites. | Boucher MJ, Ghosh S, ..., Yeh E | 10.1371/journal.pbio.2005895 |