Product Description: 26S proteasome regulatory subunit RPN12, putative
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: 3.4.25.1 (Proteasome endopeptidase complex)
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0005829 | cytosol |
| Component | GO:0005622 | intracellular |
| Component | GO:0005634 | nucleus |
| Component | GO:0005838 | proteasome regulatory particle |
| Component | GO:0008541 | proteasome regulatory particle, lid subcomplex |
| Process | GO:0043161 | proteasome-mediated ubiquitin-dependent protein catabolic process |
| Process | GO:0006511 | ubiquitin-dependent protein catabolic process |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | not expressed | N/A | N/A |
| Ring | possibly expressed | 0.12 | 0.94 |
| Trophozoite | expressed | 0.34 | 0.78 |
| Schizont | expressed | 0.24 | 0.83 |
| Gametocyte | expressed | 0.35 | 0.79 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_0312300
Resistome Missense Mutations: E260K
Resistome Compounds with Missense Mutations: MMV665794
Resistome # Samples with Disruptive Mutations: 2 (2 missense, 0 "interesting" missense)
Zhang Phenotype: Non - Mutable in CDS
MIS: 0.129 | MFS: -3.194 | #Insertions: 0
PlasmoGEM Phenotype: Slow (Pb ortholog: PBANKA_0410300)
RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PBANKA_0410300)
Modification: Disrupted | RMgm-1889
More info: PhenoPlasm Link
AlphaFill Uniprot ID: O77345
"Best" AlphaFill ligand hit: SGM (monothioglycerol, Local RMSD=0.20) with 4B0Z (Global RMSD=2.45)
BRENDA EC Inhibitors:
| EC # | Name | EC Inhibitors |
|---|---|---|
| 3.4.25.1 | proteasome endopeptidase complex | ATPSDSPSIEDTANH4+EGTATLCKALLNNMDANLVSPI 1PA28... |
No evidence of orthology to BindingDB entries
Ortholog Group (OrthoMCL): OG6_102164
Most Similar Human Ortholog: K7ERW6
TM-align score: 0.91 | RMSD: 0.88
Seq Identity: 0.47 | Length: 66 / 304
All Human Orthologs (OrthoMCL):
| Gene ID | Description |
|---|---|
| ENSG00000099341 | proteasome 26S subunit, non-ATPase 8 |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 3 | 14 | 13 | 18 |
| disruptive | 1 | 16 | 9 | 26 |
| missense | 1 | 16 | 9 | 24 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 7 | 31 | 9 | 19 |
| disruptive | 1 | 31 | 17 | 60 |
| missense | 1 | 27 | 16 | 49 |
PlasmoDB Total SNPs: 42
Non-coding: 23 | Synonymous: 18 | Nonsynonymous: 1 | Stop Codon: 0
Protein Length: 304 | Molecular Weight (kDa): 35.104
UniProt ID(s): O77345
PDB ID(s): None
Isoelectric Point: 5.06
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR006746 | 26S proteasome non-ATPase regulatory subunit Rpn12 |
| InterPro | IPR033464 | CSN8/PSMD8/EIF3K |
| PFam | PF10075 | CSN8/PSMD8/EIF3K |
| Superfamily | N/A | N/A |
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 10448855 | The complete nucleotide sequence of chromosome 3 of Plasmodium falciparum. | Bowman S, Lawson D, ..., Barrell BG | 10.1038/22964 |
| 12368867 | Sequence of Plasmodium falciparum chromosomes 1, 3-9 and 13. | Hall N, Pain A, ..., Barrell BG | 10.1038/nature01095 |
| 23181666 | Organellar proteomics reveals hundreds of novel nuclear proteins in the malariaparasite Plasmodium falciparum. | Oehring SC, Woodcroft BJ, ..., Voss TS | 10.1186/gb-2012-13-11-r108 |
| 27503796 | Proteome mapping of Plasmodium: identification of the P. yoelii remodellome. | Siau A, Huang X, ..., Preiser PR | 10.1038/srep31055 |
| 32072453 | Backbone (1)H, (13)C and (15)N resonance assignments of the proteasome lidsubunit Rpn12 from Saccharomyces cerevisiae | Niu X, Ma S, Hu Y, Jin C | 10.1007/s12104-020-09935-w |