About Gene List

PF3D7_0312500 (MFR3)

Genome location: Pf3D7_03_v3:528,316..531,025(+)

Genome classification: Core

Function and Localization

Product Description: multidrug-resistant modulator MFR3

SignalP Peptide: N/A

# Transmembrane Domains: 12

EC Numbers: None

Curated GO (PlasmoDB):

Type GO Term Name
Component GO:0005739 mitochondrion
Component GO:0005886 plasma membrane
Process GO:0009410 response to xenobiotic stimulus

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage LR class MCA mean MCA prop. zeros
Sporozoite possibly expressed N/A N/A
Ring possibly expressed 0.07 0.97
Trophozoite expressed 0.20 0.88
Schizont expressed 0.04 0.97
Gametocyte possibly expressed 0.16 0.90

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_0312500

Resistome Missense Mutations: S16R, G146R, D150V, C401Y, Q487E

Resistome Compounds with Missense Mutations: GNF-Pf-3600, MMV085203

Resistome # Samples with Disruptive Mutations: 17 (15 missense, 15 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Mutable in CDS

MIS: 1 | MFS: -1.543 | #Insertions: 3

PlasmoGEM Phenotype: Dispensable (Pb ortholog: PBANKA_0410500)

  • Relative Growth Rate: 0.97 ± 0.12
  • Confidence: 5.70

RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PbANKA_0410500)

Modification: Tagged | RMgm-4336

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: O77343

"Best" AlphaFill ligand hit: OCT (n-octane, Local RMSD=0.06) with 6JO0 (Global RMSD=19.42)

No associated EC numbers

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_533480

No human ortholog(s)

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type common rare doubleton singleton
synonymous 5 31 17 36
disruptive 12 44 35 75
missense 8 43 33 71

Any inclusion in genotype call

variant type common rare doubleton singleton
synonymous 8 56 22 48
disruptive 23 121 65 153
missense 15 107 52 105

PlasmoDB Total SNPs: 164

Non-coding: 119 | Synonymous: 29 | Nonsynonymous: 16 | Stop Codon: 0

Protein Information

Protein Length: 579 | Molecular Weight (kDa): 67.769

UniProt ID(s): O77343

PDB ID(s): None

Isoelectric Point: 8.93

Protein Domain Annotations:

Source Family ID Description
InterPro IPR011701 Major facilitator superfamily
InterPro IPR036259 MFS transporter superfamily
PFam PF07690 Major facilitator superfamily
Superfamily SSF103473 MFS transporter superfamily

Associated Publications

PMID Title Authors DOI/Link
10448855 The complete nucleotide sequence of chromosome 3 of Plasmodium falciparum. Bowman S, Lawson D, ..., Barrell BG 10.1038/22964
11152613 Predicting transmembrane protein topology with a hidden Markov model: applicationto complete genomes. Krogh A, Larsson B, von Heijne G, Sonnhammer EL 10.1006/jmbi.2000.4315
12368867 Sequence of Plasmodium falciparum chromosomes 1, 3-9 and 13. Hall N, Pain A, ..., Barrell BG 10.1038/nature01095
33715347 PfMFR3: A Multidrug-Resistant Modulator in Plasmodium falciparum. Rocamora F, Gupta P, ..., Winzeler EA 10.1021/acsinfecdis.0c00676
34756057 Characterization of Apicomplexan Amino Acid Transporters (ApiATs) in the MalariaParasite Plasmodium falciparum. Wichers JS, van Gelder C, ..., Bachmann A 10.1128/mSphere.00743-21