About Gene List

PF3D7_0313100 (HRD3)

Genome location: Pf3D7_03_v3:539,192..543,133(+)

Genome classification: Core

Function and Localization

Product Description: ubiquitin-protein ligase, putative

SignalP Peptide: MKNFMWHFVILVFIFINKKIKC

# Transmembrane Domains: 0

EC Numbers: 6.3.2.19 (Transferred entry: 2.3.2.23, 2.3.2.27 and 6.2.1.45)

Curated GO (PlasmoDB):

Type GO Term Name
Component GO:0000839 Hrd1p ubiquitin ligase ERAD-L complex
Process GO:0030433 ubiquitin-dependent ERAD pathway

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage LR class MCA mean MCA prop. zeros
Sporozoite expressed N/A N/A
Ring possibly expressed 0.36 0.83
Trophozoite expressed 1.35 0.36
Schizont expressed 0.38 0.77
Gametocyte possibly expressed 0.77 0.59

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_0313100

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Non - Mutable in CDS

MIS: 0.276 | MFS: -2.628 | #Insertions: 0

PlasmoGEM Phenotype: Essential (Pb ortholog: PBANKA_0410800)

  • Relative Growth Rate: 0.15 ± 0.16
  • Confidence: 4.99

RMgmDB ABS Phenotype: N/A

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: O77341

"Best" AlphaFill ligand hit: No AlphaFill hits

BRENDA EC Inhibitors:

EC # Name EC Inhibitors
6.3.2.19 ubiquitin-protein ligase No BRENDA inhibitors

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_137478

No human ortholog(s)

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type common rare doubleton singleton
synonymous 4 30 20 51
disruptive 21 59 33 81
missense 10 41 27 59

Any inclusion in genotype call

variant type common rare doubleton singleton
synonymous 11 67 25 56
disruptive 42 130 67 158
missense 16 84 53 113

PlasmoDB Total SNPs: 214

Non-coding: 166 | Synonymous: 31 | Nonsynonymous: 17 | Stop Codon: 0

Protein Information

Protein Length: 807 | Molecular Weight (kDa): 96.886

UniProt ID(s): O77341

PDB ID(s): None

Isoelectric Point: 9.22

Protein Domain Annotations:

Source Family ID Description
InterPro IPR006597 Sel1-like repeat
PFam PF08238 Sel1-like repeat
Superfamily SSF81901 N/A

Associated Publications

PMID Title Authors DOI/Link
10448855 The complete nucleotide sequence of chromosome 3 of Plasmodium falciparum. Bowman S, Lawson D, ..., Barrell BG 10.1038/22964
12368867 Sequence of Plasmodium falciparum chromosomes 1, 3-9 and 13. Hall N, Pain A, ..., Barrell BG 10.1038/nature01095
16267556 A protein interaction network of the malaria parasite Plasmodium falciparum. LaCount DJ, Vignali M, ..., Hughes RE 10.1038/nature04104
26184977 Analysis of antibody production in Saccharomyces cerevisiae: effects of ERprotein quality control disruption de Ruijter JC, Frey AD 10.1007/s00253-015-6807-7