Product Description: serine/threonine protein phosphatase 6, putative
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: 3.1.3.16 (Protein-serine/threonine phosphatase)
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0005737 | cytoplasm |
| Component | GO:0005634 | nucleus |
| Function | GO:0004722 | protein serine/threonine phosphatase activity |
| Process | GO:0006470 | protein dephosphorylation |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | expressed | N/A | N/A |
| Ring | expressed | 0.05 | 0.98 |
| Trophozoite | expressed | 0.05 | 0.97 |
| Schizont | expressed | 0.00 | 1.00 |
| Gametocyte | expressed | 0.04 | 0.97 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_0314400
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Non - Mutable in CDS
MIS: 0.12 | MFS: -3.016 | #Insertions: 0
PlasmoGEM Phenotype: N/A
RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PBANKA_0412100)
Modification: Tagged | RMgm-1070
More info: PhenoPlasm Link
AlphaFill Uniprot ID: O97259
"Best" AlphaFill ligand hit: NHE (2-[n-cyclohexylamino]ethane sulfonic acid, Local RMSD=0.17) with 6GHM (Global RMSD=1.35)
BRENDA EC Inhibitors:
| EC # | Name | EC Inhibitors |
|---|---|---|
| 3.1.3.16 | protein-serine/threonine phosphatase | ATPADPUDPAMPGTPUTPUMPNaFEDTAEGTAcAMPNADPH... |
Orthology to BindingDB Entries:
| UniProt | Protein Name | Species | Homology Source | BindingDB Ligands |
|---|---|---|---|---|
| P62136 | Serine/threonine-protein phosphatase PP1-alpha catalytic subunit | Homo sapiens | HOGENOM, OrthoDB | MLS001241052 |
| P36873 | Serine/threonine-protein phosphatase PP1-gamma catalytic subunit | Homo sapiens | OrthoDB | CHEMBL200117CHEMBL202197okadaic acidokadaic acid... |
| P67774 | Serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform | Bos taurus | OrthoDB | CHEMBL318253CHEMBL421377CHEMBL421377CHEMBL318253... |
| P67775 | Serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform | Homo sapiens | OrthoDB | okadaic acid analogueokadaic acid |
Ortholog Group (OrthoMCL): OG6_103044
Most Similar Human Ortholog: O00743
TM-align score: 0.97 | RMSD: 1.43
Seq Identity: 0.61 | Length: 304 / 308
All Human Orthologs (OrthoMCL):
| Gene ID | Description |
|---|---|
| ENSG00000119414 | protein phosphatase 6 catalytic subunit |
Protein Length: 308 | Molecular Weight (kDa): 35.765
UniProt ID(s): O97259
PDB ID(s): None
Isoelectric Point: 4.87
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR004843 | Calcineurin-like phosphoesterase domain, ApaH type |
| InterPro | IPR006186 | Serine/threonine-specific protein phosphatase/bis(5-nucleosyl)-tetraphosphatase |
| PFam | PF00149 | Calcineurin-like phosphoesterase domain, ApaH type |
| Superfamily | SSF56300 | N/A |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 1 | 13 | 3 | 20 |
| disruptive | 0 | 0 | 0 | 9 |
| missense | 0 | 0 | 0 | 8 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 5 | 34 | 8 | 13 |
| disruptive | 1 | 12 | 20 | 64 |
| missense | 0 | 6 | 17 | 52 |
PlasmoDB Total SNPs: 162
Non-coding: 151 | Synonymous: 9 | Nonsynonymous: 1 | Stop Codon: 1
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 10448855 | The complete nucleotide sequence of chromosome 3 of Plasmodium falciparum. | Bowman S, Lawson D, ..., Barrell BG | 10.1038/22964 |
| 12368867 | Sequence of Plasmodium falciparum chromosomes 1, 3-9 and 13. | Hall N, Pain A, ..., Barrell BG | 10.1038/nature01095 |
| 25011111 | Genome-wide functional analysis of Plasmodium protein phosphatases reveals keyregulators of parasite development and differentiation. | Guttery DS, Poulin B, ..., Tewari R | 10.1016/j.chom.2014.05.020 |
| 22177231 | Computational study of the covalent bonding of microcystins to cysteineresidues--a reaction involved in the inhibition of the PPP family of proteinphosphatases | Pereira SR, Vasconcelos VM, Antunes A | 10.1111/j.1742-4658.2011.08454.x |
| 28027965 | The serine/threonine phosphatase DhSIT4 modulates cell cycle, salt tolerance andcell wall integrity in halo tolerant yeast Debaryomyces hansenii | Chawla S, Kundu D, Randhawa A, Mondal AK | 10.1016/j.gene.2016.12.022 |