PF3D7_0316500 (NUF2)

Genome location: Pf3D7_03_v3:665,769..669,165(+)

Genome classification: Core

Function and Localization

Product Description: kinetochore protein NUF2, putative

SignalP Peptide: N/A

# Transmembrane Domains: 0

EC Numbers: None

Curated GO (PlasmoDB):

Type	GO Term	Name
Component	GO:0031262	Ndc80 complex
Component	GO:0000775	chromosome, centromeric region
Component	GO:0000778	condensed nuclear chromosome kinetochore
Component	GO:0000776	kinetochore
Component	GO:0005634	nucleus
Function	GO:0044877	protein-containing complex binding
Process	GO:0051315	attachment of mitotic spindle microtubules to kinetochore
Process	GO:0007059	chromosome segregation
Process	GO:0051383	kinetochore organization
Process	GO:0045132	meiotic chromosome segregation
Process	GO:0007052	mitotic spindle organization

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	not expressed	N/A	N/A
Ring	not expressed	0.07	0.97
Trophozoite	possibly expressed	0.50	0.71
Schizont	possibly expressed	0.57	0.66
Gametocyte	not expressed	0.75	0.59

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_0316500

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Non - Mutable in CDS

MIS: 0.202 | MFS: -3.327 | #Insertions: 0

PlasmoGEM Phenotype: Essential (Pb ortholog: PBANKA_0414300)

Relative Growth Rate: 0.19 ± 0.15
Confidence: 5.23

RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PBANKA_0414300)

Modification: Tagged | RMgm-5109

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: O77390

"Best" AlphaFill ligand hit: No AlphaFill hits

No associated EC numbers

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_143818

No human ortholog(s)

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	0	24	10	10
disruptive	2	15	9	43
missense	2	15	9	38

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	2	41	13	18
disruptive	7	48	34	90
missense	4	39	28	60

PlasmoDB Total SNPs: 164

Non-coding: 145 | Synonymous: 13 | Nonsynonymous: 6 | Stop Codon: 0

Protein Information

Protein Length: 461 | Molecular Weight (kDa): 54.677

UniProt ID(s): O77390

PDB ID(s): None

Isoelectric Point: 7.23

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR005549	Kinetochore protein Nuf2
PFam	PF03800	Kinetochore protein Nuf2
Superfamily	N/A	N/A

Associated Publications

PMID	Title	Authors	DOI/Link
10448855	The complete nucleotide sequence of chromosome 3 of Plasmodium falciparum.	Bowman S, Lawson D, ..., Barrell BG	10.1038/22964
12368867	Sequence of Plasmodium falciparum chromosomes 1, 3-9 and 13.	Hall N, Pain A, ..., Barrell BG	10.1038/nature01095
16267556	A protein interaction network of the malaria parasite Plasmodium falciparum.	LaCount DJ, Vignali M, ..., Hughes RE	10.1038/nature04104
23181666	Organellar proteomics reveals hundreds of novel nuclear proteins in the malariaparasite Plasmodium falciparum.	Oehring SC, Woodcroft BJ, ..., Voss TS	10.1186/gb-2012-13-11-r108
32501284	Real-time dynamics of Plasmodium N DC80 reveals unusual modes of chromosomesegregation during parasite proliferation	Zeeshan M, Pandey R, ..., Tewari R	10.1242/jcs.245753
36006241	Composition and organization of kinetochores show plasticity in apicomplexanchromosome segregation	Brusini L, Dos Santos Pacheco N, ..., Brochet M	10.1083/jcb.202111084
37986816	An interaction hub on Ndc80 complex facilitates dynamic recruitment of Mps1 to yeast kinetochores to promote accurate chromosome segregation	Parnell EJ, Jenson E, Miller MP	10.1101/2023.11.07.566082
36883282	Structure of the Ndc80 complex and its interactions at the yeastkinetochore-microtubule interface	Zahm JA, Jenni S, Harrison SC	10.1098/rsob.220378