Product Description: formate-nitrite transporter
SignalP Peptide: N/A
# Transmembrane Domains: 6
EC Numbers: None
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0009986 | cell surface |
| Component | GO:0005887 | integral component of plasma membrane |
| Component | GO:0005886 | plasma membrane |
| Function | GO:0015513 | high-affinity secondary active nitrite transmembrane transporter activity |
| Function | GO:0015650 | lactate:proton symporter activity |
| Process | GO:0006846 | acetate transport |
| Process | GO:0015724 | formate transport |
| Process | GO:0035873 | lactate transmembrane transport |
| Process | GO:0015707 | nitrite transport |
| Process | GO:0006848 | pyruvate transport |
| Process | GO:0009410 | response to xenobiotic stimulus |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | expressed | N/A | N/A |
| Ring | expressed | 0.17 | 0.92 |
| Trophozoite | expressed | 0.36 | 0.78 |
| Schizont | expressed | 0.09 | 0.94 |
| Gametocyte | expressed | 0.10 | 0.94 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_0316600
Resistome Missense Mutations: I92L
Resistome Compounds with Missense Mutations: Lopinavir
Resistome # Samples with Disruptive Mutations: 4 (4 missense, 4 "interesting" missense)
Zhang Phenotype: Non - Mutable in CDS
MIS: 0.869 | MFS: -2.06 | #Insertions: 0
PlasmoGEM Phenotype: N/A
RMgmDB ABS Phenotype: N/A
More info: PhenoPlasm Link
AlphaFill Uniprot ID: O77389
"Best" AlphaFill ligand hit: MA5 (cyclohexylethyl-beta-d-maltoside, Local RMSD=1.22) with 3KCU (Global RMSD=4.15)
No associated EC numbersNo evidence of orthology to BindingDB entries
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 1 | 11 | 16 | 25 |
| disruptive | 4 | 10 | 4 | 15 |
| missense | 4 | 10 | 2 | 14 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 6 | 29 | 11 | 27 |
| disruptive | 8 | 31 | 28 | 53 |
| missense | 6 | 23 | 22 | 38 |
PlasmoDB Total SNPs: 243
Non-coding: 228 | Synonymous: 9 | Nonsynonymous: 6 | Stop Codon: 0
Protein Length: 309 | Molecular Weight (kDa): 34.458
UniProt ID(s): O77389
PDB ID(s): 6VQQ, 6VQR, 7E26, 7E27, 7MXY
Isoelectric Point: 8.58
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR000292 | Formate/nitrite transporter |
| InterPro | IPR024002 | Formate/nitrite transporter, conserved site |
| PFam | PF01226 | Formate/nitrite transporter |
| Superfamily | N/A | N/A |
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 10448855 | The complete nucleotide sequence of chromosome 3 of Plasmodium falciparum. | Bowman S, Lawson D, ..., Barrell BG | 10.1038/22964 |
| 12368867 | Sequence of Plasmodium falciparum chromosomes 1, 3-9 and 13. | Hall N, Pain A, ..., Barrell BG | 10.1038/nature01095 |
| 25669138 | Identity of a Plasmodium lactate/H(+) symporter structurally unrelated to humantransporters. | Wu B, Rambow J, ..., Beitz E | 10.1038/ncomms7284 |
| 25823844 | A lactate and formate transporter in the intraerythrocytic malaria parasite,Plasmodium falciparum. | Marchetti RV, Lehane AM, ..., Kirk K | 10.1038/ncomms7721 |
| 27128092 | Interrogating the Plasmodium Sporozoite Surface: Identification ofSurface-Exposed Proteins and Demonstration of Glycosylation on CSP and TRAP byMass Spectrometry-Based Proteomics. | Swearingen KE, Lindner SE, ..., Sinnis P | 10.1371/journal.ppat.1005606 |
| 28178358 | Substrate-analogous inhibitors exert antimalarial action by targeting thePlasmodium lactate transporter PfFNT at nanomolar scale. | Golldack A, Henke B, ..., Beitz E | 10.1371/journal.ppat.1006172 |
| 28178359 | The Malaria Parasite's Lactate Transporter PfFNT Is the Target of AntiplasmodialCompounds Identified in Whole Cell Phenotypic Screens. | Hapuarachchi SV, Cobbold SA, ..., Lehane AM | 10.1371/journal.ppat.1006180 |
| 32816478 | Introduction of Scaffold Nitrogen Atoms Renders Inhibitors of the Malariall-Lactate Transporter, PfFNT, Effective against the Gly107Ser ResistanceMutation. | Walloch P, Henke B, ..., Beitz E | 10.1021/acs.jmedchem.0c00852 |
| 36857914 | Meiosis-mediated reproductive toxicity by fenitrothion in Caenorhabditis elegans from metabolomic perspective | Li W, Ma L, ..., Liu R | 10.1016/j.ecoenv.2023.114680 |
| 38159898 | Evidence for bidirectional formic acid translocation in vivo via the Escherichia coli formate channel FocA | Vanyan L, Kammel M, Sawers RG, Trchounian K | 10.1016/j.abb.2023.109877 |
| 37428074 | The Plasmodium Lactate/H(+) Transporter PfFNT Is Essential and Druggable In Vivo | Davies H, Bergmann B, ..., Beitz E | 10.1128/aac.00356-23 |