About Gene List

PF3D7_0316900

Genome location: Pf3D7_03_v3:678,813..681,861(-)

Genome classification: Core

Function and Localization

Product Description: E3 ubiquitin-protein ligase, putative

SignalP Peptide: N/A

# Transmembrane Domains: 1

EC Numbers: 6.3.2.19 (Transferred entry: 2.3.2.23, 2.3.2.27 and 6.2.1.45)

Curated GO (PlasmoDB):

Type GO Term Name
Component GO:0020011 apicoplast
Component GO:0005737 cytoplasm
Function GO:0061630 ubiquitin protein ligase activity
Function GO:0004842 ubiquitin-protein transferase activity
Function GO:0008270 zinc ion binding
Process GO:0016567 protein ubiquitination

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage LR class MCA mean MCA prop. zeros
Sporozoite expressed N/A N/A
Ring expressed 1.21 0.49
Trophozoite expressed 0.64 0.63
Schizont expressed 0.70 0.63
Gametocyte expressed 0.27 0.84

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_0316900

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Mutable in CDS

MIS: 1 | MFS: -2.411 | #Insertions: 2

PlasmoGEM Phenotype: Essential (Pb ortholog: PBANKA_0808300)

  • Relative Growth Rate: 0.16 ± 0.24
  • Confidence: 4.22

RMgmDB ABS Phenotype: N/A

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: O77387

"Best" AlphaFill ligand hit: No AlphaFill hits

BRENDA EC Inhibitors:

EC # Name EC Inhibitors
6.3.2.19 ubiquitin-protein ligase No BRENDA inhibitors

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_532418

No human ortholog(s)

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type common rare doubleton singleton
synonymous 8 27 15 28
disruptive 17 59 28 87
missense 8 27 23 63

Any inclusion in genotype call

variant type common rare doubleton singleton
synonymous 15 52 27 29
disruptive 45 142 66 160
missense 18 82 49 105

PlasmoDB Total SNPs: 265

Non-coding: 182 | Synonymous: 56 | Nonsynonymous: 27 | Stop Codon: 0

Protein Information

Protein Length: 600 | Molecular Weight (kDa): 71.009

UniProt ID(s): O77387

PDB ID(s): None

Isoelectric Point: 7.91

Protein Domain Annotations:

Source Family ID Description
InterPro IPR001841 Zinc finger, RING-type
PFam PF13920 N/A
Superfamily SSF57850 N/A

Associated Publications

PMID Title Authors DOI/Link
10448855 The complete nucleotide sequence of chromosome 3 of Plasmodium falciparum. Bowman S, Lawson D, ..., Barrell BG 10.1038/22964
12368867 Sequence of Plasmodium falciparum chromosomes 1, 3-9 and 13. Hall N, Pain A, ..., Barrell BG 10.1038/nature01095
23785288 An apicoplast localized ubiquitylation system is required for the import ofnuclear-encoded plastid proteins. Agrawal S, Chung DW, ..., Le Roch KG 10.1371/journal.ppat.1003426
30212465 Integrative proteomics and bioinformatic prediction enable a high-confidenceapicoplast proteome in malaria parasites. Boucher MJ, Ghosh S, ..., Yeh E 10.1371/journal.pbio.2005895