PF3D7_0317000

Genome location: Pf3D7_03_v3:682,236..684,432(-)

Genome classification: Core

Function and Localization

Product Description: proteasome subunit alpha type-3, putative

SignalP Peptide: N/A

# Transmembrane Domains: 0

EC Numbers: 3.4.25.1 (Proteasome endopeptidase complex)

Curated GO (PlasmoDB):

Type	GO Term	Name
Component	GO:0005737	cytoplasm
Component	GO:0005634	nucleus
Component	GO:0005839	proteasome core complex
Component	GO:0019773	proteasome core complex, alpha-subunit complex
Function	GO:0004175	endopeptidase activity
Process	GO:0010498	proteasomal protein catabolic process
Process	GO:0010499	proteasomal ubiquitin-independent protein catabolic process
Process	GO:0043161	proteasome-mediated ubiquitin-dependent protein catabolic process
Process	GO:0006511	ubiquitin-dependent protein catabolic process

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	expressed	N/A	N/A
Ring	expressed	0.17	0.92
Trophozoite	expressed	0.72	0.60
Schizont	expressed	0.33	0.79
Gametocyte	expressed	0.36	0.79

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_0317000

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Non - Mutable in CDS

MIS: 0.191 | MFS: -2.809 | #Insertions: 0

PlasmoGEM Phenotype: Essential (Pb ortholog: PBANKA_0808200)

Relative Growth Rate: 0.04 ± 0.09
Confidence: 6.21

RMgmDB ABS Phenotype: N/A

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: O77396

"Best" AlphaFill ligand hit: No AlphaFill hits

BRENDA EC Inhibitors:

EC #	Name	EC Inhibitors
3.4.25.1	proteasome endopeptidase complex	ATPSDSPSIEDTANH4+EGTATLCKALLNNMDANLVSPI 1PA28...

Orthology to BindingDB Entries:

UniProt	Protein Name	Homology Source	BindingDB Ligands
P23639	Proteasome subunit alpha type-2	OrthoDB	Carmaphycin A (1)(2S)-N-[(4S)-2,6-dimethyl-3-oxohept-1-en-4-yl]-2-[(2S)-2-hexanamido-3-methylbutanamido]-N',N'-dimethylpentanediamide (4)(2S)-N-[(4S)-2,6-dimethyl-3-oxohept-1-en-4-yl]-2-[(2S)-2-hexanamido-3-methylbutanamido]-N',N'-dimethylpentanediamide (4)Carmaphycin A (1)...
P60900	Proteasome subunit alpha type-6	OrthoDB	cid_66069med.21724, Compound 15CPZ analog, 2CPZ analog, 5...
P25786	Proteasome subunit alpha type-1	OrthoDB	CHEMBL4872258CHEMBL4877708

Orthology Information

Ortholog Group (OrthoMCL): OG6_102011

Most Similar Human Ortholog: P25788

TM-align score: 0.92 | RMSD: 1.78

Seq Identity: 0.35 | Length: 245 / 252

All Human Orthologs (OrthoMCL):

Gene ID	Description
ENSG00000100567	proteasome 20S subunit alpha 3

Protein Information

Protein Length: 252 | Molecular Weight (kDa): 29.289

UniProt ID(s): O77396

PDB ID(s): 5FMG

Isoelectric Point: 6.81

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR000426	Proteasome alpha-subunit, N-terminal domain
InterPro	IPR001353	Proteasome, subunit alpha/beta
InterPro	IPR029055	Nucleophile aminohydrolases, N-terminal
InterPro	IPR037555	Proteasome subunit alpha 3
PFam	PF00227	Proteasome, subunit alpha/beta
PFam	PF10584	Proteasome alpha-subunit, N-terminal domain
Superfamily	SSF56235	Nucleophile aminohydrolases, N-terminal

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	1	7	4	13
disruptive	0	7	4	16
missense	0	7	4	14

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	2	17	10	24
disruptive	0	21	17	65
missense	0	17	16	46

PlasmoDB Total SNPs: 130

Non-coding: 125 | Synonymous: 3 | Nonsynonymous: 2 | Stop Codon: 0

PMID	Title	Authors	DOI/Link
10448855	The complete nucleotide sequence of chromosome 3 of Plasmodium falciparum.	Bowman S, Lawson D, ..., Barrell BG	10.1038/22964
12368867	Sequence of Plasmodium falciparum chromosomes 1, 3-9 and 13.	Hall N, Pain A, ..., Barrell BG	10.1038/nature01095