PF3D7_0317100 (B9)

Genome location: Pf3D7_03_v3:686,282..689,482(+)

Genome classification: Core

Function and Localization

Product Description: 6-cysteine protein B9

SignalP Peptide: MNRGWHVVFYFILLLMNKSLG

# Transmembrane Domains: 0

EC Numbers: None

Curated GO (PlasmoDB):

Type	GO Term	Name
Component	GO:0020009	microneme
Component	GO:0005886	plasma membrane

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	expressed	N/A	N/A
Ring	possibly expressed	0.03	0.98
Trophozoite	expressed	0.06	0.97
Schizont	expressed	0.05	0.97
Gametocyte	expressed	0.14	0.92

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_0317100

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Mutable in CDS

MIS: 1 | MFS: -0.276 | #Insertions: 5

PlasmoGEM Phenotype: Dispensable (Pb ortholog: PBANKA_0808100)

Relative Growth Rate: 0.96 ± 0.17
Confidence: 4.89

RMgmDB ABS Phenotype: Not different from wild type (Pb ortholog: PBANKA_0808100)

Modification: Disrupted | RMgm-5267

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: C0H478

"Best" AlphaFill ligand hit: SAH (s-adenosyl-l-homocysteine, Local RMSD=16.13) with 2OB2 (Global RMSD=15.78)

No associated EC numbers

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_533665

No human ortholog(s)

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	9	42	28	56
disruptive	24	80	44	130
missense	17	69	39	104

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	13	87	45	58
disruptive	49	175	95	221
missense	29	135	75	147

PlasmoDB Total SNPs: 120

Non-coding: 44 | Synonymous: 49 | Nonsynonymous: 27 | Stop Codon: 0

Protein Information

Protein Length: 969 | Molecular Weight (kDa): 114.735

UniProt ID(s): C0H478

PDB ID(s): None

Isoelectric Point: 6.7

Protein Domain Annotations:

Source	Family ID	Description
InterPro	N/A	N/A
PFam	N/A	N/A
Superfamily	N/A	N/A

PMID	Title	Authors	DOI/Link
10448855	The complete nucleotide sequence of chromosome 3 of Plasmodium falciparum.	Bowman S, Lawson D, ..., Barrell BG	10.1038/22964
12368867	Sequence of Plasmodium falciparum chromosomes 1, 3-9 and 13.	Hall N, Pain A, ..., Barrell BG	10.1038/nature01095
24509910	Two Plasmodium 6-Cys family-related proteins have distinct and critical roles inliver-stage development.	Annoura T, van Schaijk BC, ..., Khan SM	10.1096/fj.13-241570