PF3D7_0317400

Genome location: Pf3D7_03_v3:711,998..714,150(-)

Genome classification: Core

Function and Localization

Product Description: DNA replication complex GINS protein, putative

SignalP Peptide: N/A

# Transmembrane Domains: 0

EC Numbers: None

Curated GO (PlasmoDB):

Type	GO Term	Name
Component	GO:0000811	GINS complex
Component	GO:0031298	replication fork protection complex
Process	GO:0000727	double-strand break repair via break-induced replication

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	not expressed	N/A	N/A
Ring	not expressed	0.09	0.96
Trophozoite	expressed	0.11	0.92
Schizont	possibly expressed	0.09	0.93
Gametocyte	possibly expressed	0.07	0.96

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_0317400

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Non - Mutable in CDS

MIS: 0.127 | MFS: -2.853 | #Insertions: 0

PlasmoGEM Phenotype: Dispensable (Pb ortholog: PBANKA_1349400)

Relative Growth Rate: 1.01 ± 0.13
Confidence: 5.41

RMgmDB ABS Phenotype: N/A

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: O77383

"Best" AlphaFill ligand hit: GOX ((2s,3s,4r,5r)-6-(hydroxyamino)-2-(hydroxymethyl)-2,3,4,5-tetrahydropyridine-3,4,5-triol, Local RMSD=0.70) with 1E6S (Global RMSD=20.93)

No associated EC numbers

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_145254

No human ortholog(s)

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	5	39	19	29
disruptive	1	29	9	59
missense	1	22	8	40

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	10	72	16	36
disruptive	9	100	47	182
missense	3	69	30	79

PlasmoDB Total SNPs: 126

Non-coding: 97 | Synonymous: 26 | Nonsynonymous: 3 | Stop Codon: 0

Protein Information

Protein Length: 518 | Molecular Weight (kDa): 61.493

UniProt ID(s): O77383

PDB ID(s): None

Isoelectric Point: 7.61

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR007257	DNA replication complex GINS protein Psf2
InterPro	IPR021151	GINS subunit, domain A
InterPro	IPR036224	GINS, helical bundle-like domain superfamily
PFam	PF05916	GINS subunit, domain A
Superfamily	SSF158573	GINS, helical bundle-like domain superfamily

PMID	Title	Authors	DOI/Link
10448855	The complete nucleotide sequence of chromosome 3 of Plasmodium falciparum.	Bowman S, Lawson D, ..., Barrell BG	10.1038/22964
12368867	Sequence of Plasmodium falciparum chromosomes 1, 3-9 and 13.	Hall N, Pain A, ..., Barrell BG	10.1038/nature01095