PF3D7_0317700

Genome location: Pf3D7_03_v3:727,544..738,158(+)

Genome classification: Core

Function and Localization

Product Description: CPSF (cleavage and polyadenylation specific factor), subunit A, putative

SignalP Peptide: N/A

# Transmembrane Domains: 0

EC Numbers: None

Curated GO (PlasmoDB):

Type	GO Term	Name
Component	GO:0031461	cullin-RING ubiquitin ligase complex
Component	GO:0005634	nucleus
Function	GO:0003684	damaged DNA binding
Process	GO:0006289	nucleotide-excision repair
Process	GO:0043161	proteasome-mediated ubiquitin-dependent protein catabolic process

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	not expressed	N/A	N/A
Ring	possibly expressed	0.66	0.70
Trophozoite	expressed	0.97	0.51
Schizont	expressed	0.49	0.73
Gametocyte	not expressed	1.64	0.28

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_0317700

Resistome Missense Mutations: L99F

Resistome Compounds with Missense Mutations: AN13956

Resistome # Samples with Disruptive Mutations: 2 (2 missense, 2 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Non - Mutable in CDS

MIS: 0.408 | MFS: -2.51 | #Insertions: 0

PlasmoGEM Phenotype: Essential (Pb ortholog: PBANKA_0807500)

Relative Growth Rate: 0.34 ± 0.60
Confidence: 2.40

RMgmDB ABS Phenotype: N/A

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: N/A

"Best" AlphaFill ligand hit: No AlphaFill hits

No associated EC numbers

Orthology to BindingDB Entries:

UniProt	Protein Name	Species	Homology Source	BindingDB Ligands
Q16531	DNA damage-binding protein 1/Protein cereblon		OrthoDB	US9694015, 6.1US9694015, 6.2US9694015, 6.1US9694015, 6.2...

Orthology Information

Ortholog Group (OrthoMCL): OG6_185275

No human ortholog(s)

Protein Information

Protein Length: 2870 | Molecular Weight (kDa): 338.532

UniProt ID(s): C7DU24, O77380, Q8IFS8, Q9U0N9

PDB ID(s): None

Isoelectric Point: 7.72

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR004871	Cleavage/polyadenylation specificity factor, A subunit, C-terminal
InterPro	IPR031297	DNA damage-binding protein 1
PFam	PF03178	Cleavage/polyadenylation specificity factor, A subunit, C-terminal
Superfamily	SSF69349	N/A

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	21	141	79	161
disruptive	115	287	195	398
missense	60	223	162	314

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	43	263	116	178
disruptive	216	604	306	601
missense	95	465	249	422

PlasmoDB Total SNPs: 484

Non-coding: 144 | Synonymous: 215 | Nonsynonymous: 123 | Stop Codon: 2

PMID	Title	Authors	DOI/Link
10448855	The complete nucleotide sequence of chromosome 3 of Plasmodium falciparum.	Bowman S, Lawson D, ..., Barrell BG	10.1038/22964
12368867	Sequence of Plasmodium falciparum chromosomes 1, 3-9 and 13.	Hall N, Pain A, ..., Barrell BG	10.1038/nature01095