About Gene List

PF3D7_0318800

Genome location: Pf3D7_03_v3:789,497..791,504(+)

Genome classification: Core

Function and Localization

Product Description: triosephosphate isomerase, putative

SignalP Peptide: MLKTLVLLSFYSLYTVFQ

# Transmembrane Domains: 0

EC Numbers: 5.3.1.1 (Triose-phosphate isomerase)

Curated GO (PlasmoDB):

Type GO Term Name
Component GO:0020011 apicoplast
Component GO:0005829 cytosol
Function GO:0004807 triose-phosphate isomerase activity
Process GO:0006094 gluconeogenesis
Process GO:0046166 glyceraldehyde-3-phosphate biosynthetic process
Process GO:0019563 glycerol catabolic process
Process GO:0006096 glycolytic process

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage LR class MCA mean MCA prop. zeros
Sporozoite not expressed N/A N/A
Ring not expressed 0.03 0.99
Trophozoite not expressed 0.01 0.99
Schizont not expressed 0.02 0.99
Gametocyte not expressed 0.17 0.90

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_0318800

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Mutable in CDS

MIS: 0.799 | MFS: -2.996 | #Insertions: 1

PlasmoGEM Phenotype: Dispensable (Pb ortholog: PBANKA_0806500)

  • Relative Growth Rate: 1.04 ± 0.09
  • Confidence: 6.15

RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PBANKA_0806500)

Modification: Disrupted | RMgm-2320

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: O77370

"Best" AlphaFill ligand hit: 2PG (2-phosphoglyceric acid, Local RMSD=0.12) with 3UWV (Global RMSD=2.78)

BRENDA EC Inhibitors:

EC # Name EC Inhibitors
5.3.1.1 triose-phosphate isomerase ATPNEMPCMBGSSGSO42-AsO2-AsO43-citratesuraminDiamidearsenateAtrazine...

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_186486

No human ortholog(s)

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type common rare doubleton singleton
synonymous 2 12 13 31
disruptive 6 31 26 40
missense 5 27 21 37

Any inclusion in genotype call

variant type common rare doubleton singleton
synonymous 4 37 18 25
disruptive 14 67 37 87
missense 9 55 31 66

PlasmoDB Total SNPs: 158

Non-coding: 112 | Synonymous: 35 | Nonsynonymous: 9 | Stop Codon: 2

Protein Information

Protein Length: 357 | Molecular Weight (kDa): 41.578

UniProt ID(s): O77370

PDB ID(s): None

Isoelectric Point: 9.57

Protein Domain Annotations:

Source Family ID Description
InterPro IPR000652 Triosephosphate isomerase
InterPro IPR035990 Triosephosphate isomerase superfamily
PFam PF00121 Triosephosphate isomerase
Superfamily SSF51351 Triosephosphate isomerase superfamily

Associated Publications

PMID Title Authors DOI/Link
10448855 The complete nucleotide sequence of chromosome 3 of Plasmodium falciparum. Bowman S, Lawson D, ..., Barrell BG 10.1038/22964
12368867 Sequence of Plasmodium falciparum chromosomes 1, 3-9 and 13. Hall N, Pain A, ..., Barrell BG 10.1038/nature01095
15465054 Structures of unliganded and inhibitor complexes of W168F, a Loop6 hinge mutantof Plasmodium falciparum triosephosphate isomerase: observation of anintermediate position of loop6. Eaazhisai K, Balaram H, Balaram P, Murthy MR 10.1016/j.jmb.2004.08.060