Genome location: Pf3D7_03_v3:794,521..801,871(+)
Genome classification: Core
Product Description: P4-type ATPase ATP7, putative
SignalP Peptide: N/A
# Transmembrane Domains: 10
EC Numbers: 3.6.3.1 (Transferred entry: 7.6.2.1);7.6.2.1 (P-type phospholipid transporter)
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0005737 | cytoplasm |
| Component | GO:0005887 | integral component of plasma membrane |
| Component | GO:0005886 | plasma membrane |
| Function | GO:0140326 | ATPase-coupled intramembrane lipid transporter activity |
| Function | GO:0015662 | P-type ion transporter activity |
| Function | GO:0140345 | phosphatidylcholine flippase activity |
| Process | GO:0006812 | cation transport |
| Process | GO:0045332 | phospholipid translocation |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | not expressed | N/A | N/A |
| Ring | not expressed | 0.09 | 0.96 |
| Trophozoite | expressed | 0.59 | 0.68 |
| Schizont | expressed | 1.05 | 0.48 |
| Gametocyte | expressed | 0.47 | 0.74 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_0319000
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 4 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Mutable in CDS
MIS: 1 | MFS: -0.599 | #Insertions: 9
PlasmoGEM Phenotype: Dispensable (Pb ortholog: PBANKA_0806300)
RMgmDB ABS Phenotype: Not tested (Pb ortholog: PY17X_0711800)
Modification: Disrupted | RMgm-5050
More info: PhenoPlasm Link
AlphaFill Uniprot ID: O77368
"Best" AlphaFill ligand hit: P5S (phosphatidyl serine, Local RMSD=0.14) with 6LKN (Global RMSD=2.72)
BRENDA EC Inhibitors:
No evidence of orthology to BindingDB entries
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 12 | 62 | 49 | 96 |
| disruptive | 30 | 85 | 59 | 133 |
| missense | 20 | 71 | 47 | 100 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 30 | 146 | 68 | 107 |
| disruptive | 60 | 225 | 129 | 377 |
| missense | 34 | 156 | 98 | 224 |
PlasmoDB Total SNPs: 216
Non-coding: 120 | Synonymous: 66 | Nonsynonymous: 27 | Stop Codon: 3
Protein Length: 1864 | Molecular Weight (kDa): 218.02
UniProt ID(s): O77368
PDB ID(s): None
Isoelectric Point: 8.07
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR001757 | P-type ATPase |
| InterPro | IPR006539 | P-type ATPase, subfamily IV |
| InterPro | IPR008250 | P-type ATPase, A domain superfamily |
| InterPro | IPR018303 | P-type ATPase, phosphorylation site |
| InterPro | IPR023298 | P-type ATPase, transmembrane domain superfamily |
| InterPro | IPR023299 | P-type ATPase, cytoplasmic domain N |
| InterPro | IPR032630 | P-type ATPase, C-terminal |
| InterPro | IPR032631 | P-type ATPase, N-terminal |
| InterPro | IPR036412 | HAD-like superfamily |
| PFam | PF13246 | P-type ATPase, N-terminal |
| PFam | PF16209 | P-type ATPase, C-terminal |
| PFam | PF16212 | |
| Superfamily | SSF56784 | HAD-like superfamily |
| Superfamily | SSF81653 | P-type ATPase, A domain superfamily |
| Superfamily | SSF81660 | P-type ATPase, cytoplasmic domain N |
| Superfamily | SSF81665 | P-type ATPase, transmembrane domain superfamily |
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 10448855 | The complete nucleotide sequence of chromosome 3 of Plasmodium falciparum. | Bowman S, Lawson D, ..., Barrell BG | 10.1038/22964 |
| 12368867 | Sequence of Plasmodium falciparum chromosomes 1, 3-9 and 13. | Hall N, Pain A, ..., Barrell BG | 10.1038/nature01095 |
| 34301597 | A malaria parasite phospholipid flippase safeguards midgut traversal of ookinetesfor mosquito transmission | Yang Z, Shi Y, ..., Yuan J | 10.1126/sciadv.abf6015 |
| 9520266 | Isolation and characterization of hrp1+, a new member of the SNF2/SWI2 genefamily from the fission yeast Schizosaccharomyces pombe | Jin YH, Yoo EJ, ..., Park SD | 10.1007/s004380050653 |