Product Description: E3 ubiquitin-protein ligase RBX1, putative
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: 6.3.2.19 (Transferred entry: 2.3.2.23, 2.3.2.27 and 6.2.1.45)
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0019005 | SCF ubiquitin ligase complex |
| Component | GO:0031461 | cullin-RING ubiquitin ligase complex |
| Component | GO:0005634 | nucleus |
| Component | GO:0000151 | ubiquitin ligase complex |
| Function | GO:0097602 | cullin family protein binding |
| Function | GO:0061630 | ubiquitin protein ligase activity |
| Function | GO:0004842 | ubiquitin-protein transferase activity |
| Process | GO:0016567 | protein ubiquitination |
| Process | GO:0006511 | ubiquitin-dependent protein catabolic process |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | expressed | N/A | N/A |
| Ring | expressed | 0.03 | 0.98 |
| Trophozoite | expressed | 0.16 | 0.89 |
| Schizont | expressed | 0.07 | 0.94 |
| Gametocyte | expressed | 0.12 | 0.92 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_0319100
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Non - Mutable in CDS *tentative
MIS: 0.987 | MFS: -1.971 | #Insertions: 0
PlasmoGEM Phenotype: Essential (Pb ortholog: PBANKA_0806200)
RMgmDB ABS Phenotype: Not different from wild type (Pb ortholog: PBANKA_0806200)
Modification: Mutated | RMgm-5339
More info: PhenoPlasm Link
AlphaFill Uniprot ID: O77367
"Best" AlphaFill ligand hit: No AlphaFill hits
BRENDA EC Inhibitors:
| EC # | Name | EC Inhibitors |
|---|---|---|
| 6.3.2.19 | ubiquitin-protein ligase | No BRENDA inhibitors |
No evidence of orthology to BindingDB entries
Ortholog Group (OrthoMCL): OG6_102466
Most Similar Human Ortholog: P62877
TM-align score: 0.67 | RMSD: 1.58
Seq Identity: 0.73 | Length: 78 / 107
All Human Orthologs (OrthoMCL):
| Gene ID | Description |
|---|---|
| ENSG00000100387 | ring-box 1 |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 0 | 3 | 4 | 3 |
| disruptive | 0 | 1 | 3 | 7 |
| missense | 0 | 1 | 2 | 6 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 0 | 5 | 3 | 9 |
| disruptive | 1 | 5 | 8 | 17 |
| missense | 0 | 4 | 7 | 12 |
PlasmoDB Total SNPs: 123
Non-coding: 116 | Synonymous: 7 | Nonsynonymous: 0 | Stop Codon: 0
Protein Length: 107 | Molecular Weight (kDa): 12.419
UniProt ID(s): O77367
PDB ID(s): None
Isoelectric Point: 6.72
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR024766 | Zinc finger, RING-H2-type |
| PFam | PF12678 | Zinc finger, RING-H2-type |
| Superfamily | SSF57850 | N/A |
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 10448855 | The complete nucleotide sequence of chromosome 3 of Plasmodium falciparum. | Bowman S, Lawson D, ..., Barrell BG | 10.1038/22964 |
| 12368867 | Sequence of Plasmodium falciparum chromosomes 1, 3-9 and 13. | Hall N, Pain A, ..., Barrell BG | 10.1038/nature01095 |
| 38416790 | Plasmodium falciparum contains functional SCF and CRL4 ubiquitin E3 ligases, andCRL4 is critical for cell division and membrane integrity | Rizvi Z, Reddy GS, ..., Sijwali PS | 10.1371/journal.ppat.1012045 |