Product Description: ubiquitin-conjugating enzyme E2, putative
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: 2.3.2.23 (E2 ubiquitin-conjugating enzyme)
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Function | GO:0061631 | ubiquitin conjugating enzyme activity |
| Function | GO:0004842 | ubiquitin-protein transferase activity |
| Process | GO:0006281 | DNA repair |
| Process | GO:0016574 | histone ubiquitination |
| Process | GO:0043161 | proteasome-mediated ubiquitin-dependent protein catabolic process |
| Process | GO:0000209 | protein polyubiquitination |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | possibly expressed | N/A | N/A |
| Ring | expressed | 0.03 | 0.98 |
| Trophozoite | expressed | 0.07 | 0.95 |
| Schizont | expressed | 0.01 | 0.99 |
| Gametocyte | expressed | 0.03 | 0.98 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_0319300
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Non - Mutable in CDS *tentative
MIS: 0.986 | MFS: -1.899 | #Insertions: 0
PlasmoGEM Phenotype: Dispensable (Pb ortholog: PBANKA_0806000)
RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PBANKA_0806000)
Modification: Disrupted | RMgm-2315
More info: PhenoPlasm Link
AlphaFill Uniprot ID: O77397
"Best" AlphaFill ligand hit: NHE (2-[n-cyclohexylamino]ethane sulfonic acid, Local RMSD=0.54) with 4FH1 (Global RMSD=1.88)
BRENDA EC Inhibitors:
| EC # | Name | EC Inhibitors |
|---|---|---|
| 2.3.2.23 | E2 ubiquitin-conjugating enzyme | CC0651[1,1'-biphenyl]-2-ol[1,1'-biphenyl]-2,2'-diol4-phenyl-1,3-thiazol-2-amine[1,1'-biphenyl]-2-sulfonamide5-chloro-1,3-benzoxazole-2-thiol6-chloro-1,3-benzoxazole-2-thiol1-(1,3-benzothiazol-2-yl)methanamine2-amino-5-phenylfuran-3-carbonitrile3-(2-hydroxyphenyl)pyridin-2(1H)-one3-methyl-3,4-dihydroquinazolin-2(1H)-one5,6-dibenzo[c,e][1,2]thiazine-5,5(6H)-dione... |
Orthology to BindingDB Entries:
| UniProt | Protein Name | Species | Homology Source | BindingDB Ligands |
|---|---|---|---|---|
| P63146 | Ubiquitin-conjugating enzyme E2 B | HOGENOM, OMA, OrthoDB | CHEMBL3787660 | |
| P51965 | Ubiquitin-conjugating enzyme E2 E1 | OrthoDB | US9796725, Compound I-001US9796725, Compound I-098US9796725, Compound I-003US9796725, Compound I-004... | |
| P61088 | Ubiquitin-conjugating enzyme E2 N | Homo sapiens | OrthoDB | MLS000074439MLS000032400MLS000041097MLS000055488... |
Ortholog Group (OrthoMCL): OG6_101178
Most Similar Human Ortholog: A0A0D9SG71
TM-align score: 0.97 | RMSD: 0.94
Seq Identity: 0.57 | Length: 119 / 160
All Human Orthologs (OrthoMCL):
| Gene ID | Description |
|---|---|
| ENSG00000077721 | ubiquitin conjugating enzyme E2 A |
| ENSG00000119048 | ubiquitin conjugating enzyme E2 B |
Protein Length: 160 | Molecular Weight (kDa): 18.627
UniProt ID(s): O77397
PDB ID(s): None
Isoelectric Point: 6.84
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR000608 | Ubiquitin-conjugating enzyme E2 |
| InterPro | IPR016135 | Ubiquitin-conjugating enzyme/RWD-like |
| InterPro | IPR023313 | Ubiquitin-conjugating enzyme, active site |
| PFam | PF00179 | Ubiquitin-conjugating enzyme E2 |
| Superfamily | SSF54495 | Ubiquitin-conjugating enzyme/RWD-like |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 1 | 8 | 3 | 13 |
| disruptive | 0 | 1 | 2 | 11 |
| missense | 0 | 1 | 2 | 9 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 5 | 19 | 5 | 9 |
| disruptive | 1 | 13 | 26 | 38 |
| missense | 1 | 11 | 24 | 27 |
PlasmoDB Total SNPs: 68
Non-coding: 64 | Synonymous: 3 | Nonsynonymous: 1 | Stop Codon: 0
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 10448855 | The complete nucleotide sequence of chromosome 3 of Plasmodium falciparum. | Bowman S, Lawson D, ..., Barrell BG | 10.1038/22964 |
| 12368867 | Sequence of Plasmodium falciparum chromosomes 1, 3-9 and 13. | Hall N, Pain A, ..., Barrell BG | 10.1038/nature01095 |