Product Description: nicotinamidase, putative
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: 3.5.1.19 (Nicotinamidase)
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0005737 | cytoplasm |
| Component | GO:0005634 | nucleus |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | not expressed | N/A | N/A |
| Ring | expressed | 0.11 | 0.94 |
| Trophozoite | expressed | 0.31 | 0.82 |
| Schizont | expressed | 1.11 | 0.48 |
| Gametocyte | expressed | 0.26 | 0.85 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_0320500
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Non - Mutable in CDS
MIS: 0.145 | MFS: -3.167 | #Insertions: 0
PlasmoGEM Phenotype: Dispensable (Pb ortholog: PBANKA_1218000)
RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PBANKA_1218000)
Modification: Disrupted | RMgm-3263
More info: PhenoPlasm Link
AlphaFill Uniprot ID: O97284
"Best" AlphaFill ligand hit: NIO (nicotinic acid, Local RMSD=0.41) with 2WT9 (Global RMSD=2.35)
BRENDA EC Inhibitors:
| EC # | Name | EC Inhibitors |
|---|---|---|
| 3.5.1.19 | nicotinamidase | CysNAD+EDTAPCMBHgCl2ZnCl2CuCl2NiCl2isoniazidL-thyroxineTriton X-100benzaldehyde... |
Orthology to BindingDB Entries:
| UniProt | Protein Name | Species | Homology Source | BindingDB Ligands |
|---|---|---|---|---|
| Q7YTI0 | Isochorismatase domain-containing protein | Caenorhabditis elegans | OrthoDB, OrthoMCL | Nicotinamidase Inhibitor, 16Nicotinamidase Inhibitor, 17Nicotinamidase Inhibitor, 18Nicotinamidase Inhibitor, 22 |
| P53184 | Nicotinamidase | Saccharomyces cerevisiae | OrthoDB, OrthoMCL | Nicotinamidase Inhibitor, 16Nicotinamidase Inhibitor, 17Nicotinamidase Inhibitor, 18Nicotinamidase Inhibitor, 19 |
| Q9N426 | Isochorismatase domain-containing protein | Caenorhabditis elegans | OrthoDB, OrthoMCL | Nicotinamidase Inhibitor, 16Nicotinamidase Inhibitor, 17Nicotinamidase Inhibitor, 18Nicotinamidase Inhibitor, 22 |
| O50718 | Pyrazinamidase/nicotinamidase, PncA protein | Borrelia burgdorferi | OrthoMCL BLAST | Nicotinamidase Inhibitor, 16Nicotinamidase Inhibitor, 17Nicotinamidase Inhibitor, 18Nicotinamidase Inhibitor, 19 |
Protein Length: 429 | Molecular Weight (kDa): 50.151
UniProt ID(s): O97284
PDB ID(s): None
Isoelectric Point: 5.22
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR000868 | Isochorismatase-like |
| InterPro | IPR036380 | Isochorismatase-like superfamily |
| PFam | PF00857 | Isochorismatase-like |
| Superfamily | SSF52499 | Isochorismatase-like superfamily |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 2 | 14 | 6 | 25 |
| disruptive | 7 | 22 | 29 | 52 |
| missense | 6 | 21 | 27 | 47 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 2 | 30 | 17 | 24 |
| disruptive | 10 | 78 | 57 | 114 |
| missense | 8 | 65 | 49 | 70 |
PlasmoDB Total SNPs: 146
Non-coding: 104 | Synonymous: 31 | Nonsynonymous: 11 | Stop Codon: 0
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 10448855 | The complete nucleotide sequence of chromosome 3 of Plasmodium falciparum. | Bowman S, Lawson D, ..., Barrell BG | 10.1038/22964 |
| 12368867 | Sequence of Plasmodium falciparum chromosomes 1, 3-9 and 13. | Hall N, Pain A, ..., Barrell BG | 10.1038/nature01095 |
| 24747974 | Targeting NAD+ metabolism in the human malaria parasite Plasmodium falciparum. | O'Hara JK, Kerwin LJ, ..., Llinas M | 10.1371/journal.pone.0094061 |