Product Description: ATP-dependent RNA helicase DDX6
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: 3.6.4.13 (RNA helicase)
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0000932 | P-body |
| Component | GO:0005737 | cytoplasm |
| Component | GO:0010494 | cytoplasmic stress granule |
| Component | GO:0005634 | nucleus |
| Function | GO:0003724 | RNA helicase activity |
| Function | GO:0003729 | mRNA binding |
| Function | GO:0005515 | protein binding |
| Process | GO:0033962 | P-body assembly |
| Process | GO:0034063 | stress granule assembly |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | expressed | N/A | N/A |
| Ring | expressed | 0.05 | 0.98 |
| Trophozoite | expressed | 0.19 | 0.87 |
| Schizont | expressed | 0.19 | 0.88 |
| Gametocyte | expressed | 0.34 | 0.80 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_0320800
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Mutable in CDS
MIS: 1 | MFS: -1.795 | #Insertions: 2
PlasmoGEM Phenotype: Dispensable (Pb ortholog: PBANKA_1217700)
RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PBANKA_1217700)
Modification: Disrupted | RMgm-3260
More info: PhenoPlasm Link
AlphaFill Uniprot ID: O97285
"Best" AlphaFill ligand hit: AGS (phosphothiophosphoric acid-adenylate ester, Local RMSD=0.08) with 1OYY (Global RMSD=2.16)
BRENDA EC Inhibitors:
| EC # | Name | EC Inhibitors |
|---|---|---|
| 3.6.4.13 | RNA helicase | ATPADPUDPAMPGTPGDPUTPKClNEMCDPITPTDP... |
Orthology to BindingDB Entries:
Ortholog Group (OrthoMCL): OG6_101887
Most Similar Human Ortholog: P26196
TM-align score: 0.93 | RMSD: 1.20
Seq Identity: 0.68 | Length: 408 / 433
All Human Orthologs (OrthoMCL):
| Gene ID | Description |
|---|---|
| ENSG00000110367 | DEAD-box helicase 6 |
Protein Length: 433 | Molecular Weight (kDa): 49.413
PDB ID(s): None
Isoelectric Point: 8.04
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR000629 | ATP-dependent RNA helicase DEAD-box, conserved site |
| InterPro | IPR001650 | Helicase, C-terminal |
| InterPro | IPR011545 | DEAD/DEAH box helicase domain |
| InterPro | IPR014001 | Helicase superfamily 1/2, ATP-binding domain |
| InterPro | IPR027417 | P-loop containing nucleoside triphosphate hydrolase |
| PFam | PF00270 | DEAD/DEAH box helicase domain |
| PFam | PF00271 | Helicase, C-terminal |
| Superfamily | SSF52540 | P-loop containing nucleoside triphosphate hydrolase |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 7 | 19 | 11 | 10 |
| disruptive | 0 | 1 | 2 | 10 |
| missense | 0 | 1 | 2 | 8 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 12 | 33 | 18 | 17 |
| disruptive | 0 | 20 | 28 | 87 |
| missense | 0 | 15 | 22 | 58 |
PlasmoDB Total SNPs: 185
Non-coding: 177 | Synonymous: 8 | Nonsynonymous: 0 | Stop Codon: 0
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 10448855 | The complete nucleotide sequence of chromosome 3 of Plasmodium falciparum. | Bowman S, Lawson D, ..., Barrell BG | 10.1038/22964 |
| 12368867 | Sequence of Plasmodium falciparum chromosomes 1, 3-9 and 13. | Hall N, Pain A, ..., Barrell BG | 10.1038/nature01095 |
| 20169188 | Universal features of post-transcriptional gene regulation are critical forPlasmodium zygote development. | Mair GR, Lasonder E, ..., Waters AP | 10.1371/journal.ppat.1000767 |
| 23181666 | Organellar proteomics reveals hundreds of novel nuclear proteins in the malariaparasite Plasmodium falciparum. | Oehring SC, Woodcroft BJ, ..., Voss TS | 10.1186/gb-2012-13-11-r108 |
| 23562722 | Plasmodium falciparum DOZI, an RNA helicase interacts with eIF4E. | Tarique M, Ahmad M, Ansari A, Tuteja R | 10.1016/j.gene.2013.03.063 |
| 27381095 | The mRNA-bound proteome of the human malaria parasite Plasmodium falciparum. | Bunnik EM, Batugedara G, ..., Le Roch KG | 10.1186/s13059-016-1014-0 |
| 35947628 | Identification of a novel AP2 transcription factor in zygotes with an essentialrole in Plasmodium ookinete development | Nishi T, Kaneko I, Iwanaga S, Yuda M | 10.1371/journal.ppat.1010510 |
| 32817449 | Redesigned TetR-Aptamer System To Control Gene Expression in Plasmodiumfalciparum | Rajaram K, Liu HB, Prigge ST | 10.1128/mSphere.00457-20 |
| 25387887 | Translational regulation during stage transitions in malaria parasites | Cui L, Lindner S, Miao J | 10.1111/nyas.12573 |
| 34604117 | Roles and Cellular Localization of GBP2 and NAB2 During the Blood Stage ofMalaria Parasites | Niikura M, Fukutomi T, Mitobe J, Kobayashi F | 10.3389/fcimb.2021.737457 |
| 26525978 | A bioinformatic survey of RNA-binding proteins in Plasmodium | Reddy BP, Shrestha S, ..., Lindner SE | 10.1186/s12864-015-2092-1 |
| 31990132 | EXP1 is required for organisation of EXP2 in the intraerythrocytic malariaparasite vacuole | Nessel T, Beck JM, ..., Beck JR | 10.1111/cmi.13168 |
| 25418785 | Genome-wide RIP-Chip analysis of translational repressor-bound mRNAs in thePlasmodium gametocyte | Guerreiro A, Deligianni E, ..., Mair GR | 10.1186/s13059-014-0493-0 |
| 34973333 | Enzymatic and structural characterization of HAD5, an essentialphosphomannomutase of malaria-causing parasites | Frasse PM, Miller JJ, ..., Odom John AR | 10.1016/j.jbc.2021.101550 |
| 24709106 | Characterization of a homolog of DEAD-box RNA helicases in Toxoplasma gondii as amarker of cytoplasmic mRNP stress granules | Cherry AA, Ananvoranich S | 10.1016/j.gene.2014.04.011 |
| 29626096 | The mitochondrial ribosomal protein L13 is critical for the structural andfunctional integrity of the mitochondrion in Plasmodium falciparum | Ke H, Dass S, ..., Vaidya AB | 10.1074/jbc.RA118.002552 |
| 37702516 | Plasmodium falciparum GCN5 plays a key role in regulating artemisininresistance-related stress responses | Lucky AB, Wang C, ..., Miao J | 10.1128/aac.00577-23 |
| 27303037 | Maternally supplied S-acyl-transferase is required for crystalloid organelleformation and transmission of the malaria parasite | Santos JM, Duarte N, ..., Mair GR | https://pubmed.ncbi.nlm.nih.gov/27303037/ |
| 38352447 | Global Release of Translational Repression Across Plasmodium's Host-to-VectorTransmission Event | Rios KT, McGee JP, ..., Lindner SE | 10.1101/2024.02.01.577866 |
| 30133543 | A seven-helix protein constitutes stress granules crucial for regulatingtranslation during human-to-mosquito transmission of Plasmodium falciparum | Bennink S, von Bohl A, ..., Pradel G | 10.1371/journal.ppat.1007249 |
| 33347893 | Gliding motility protein LIMP promotes optimal mosquito midgut traversal andinfection by Plasmodium berghei | Egarter S, Santos JM, ..., Mair GR | 10.1016/j.molbiopara.2020.111347 |
| 27312996 | Translational repression of the cpw-wpc gene family in the malaria parasitePlasmodium | Rao PN, Santos JM, ..., Mair GR | 10.1016/j.parint.2016.06.007 |
| 36711954 | Plasmodium falciparum GCN5 plays a key role in regulating artemisininresistance-related stress responses | Lucky AB, Wang C, ..., Miao J | 10.1101/2023.01.11.523703 |