Product Description: UDP-N-acetylglucosamine--dolichyl-phosphate N-acetylglucosaminephosphotransferase, putative
SignalP Peptide: N/A
# Transmembrane Domains: 12
EC Numbers: 2.7.8.13 (Phospho-N-acetylmuramoyl-pentapeptide-transferase);2.7.8.15 (UDP-N-acetylglucosamine--dolichyl-phosphateN-acetylglucosaminephosphotransferase)
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0043541 | UDP-N-acetylglucosamine transferase complex |
| Component | GO:0016021 | integral component of membrane |
| Function | GO:0003975 | UDP-N-acetylglucosamine-dolichyl-phosphate N-acetylglucosaminephosphotransferase activity |
| Process | GO:0006488 | dolichol-linked oligosaccharide biosynthetic process |
| Process | GO:0006489 | dolichyl diphosphate biosynthetic process |
| Process | GO:0006487 | protein N-linked glycosylation |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | possibly expressed | N/A | N/A |
| Ring | expressed | 0.01 | 0.99 |
| Trophozoite | expressed | 0.06 | 0.96 |
| Schizont | expressed | 0.02 | 0.99 |
| Gametocyte | expressed | 0.06 | 0.96 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_0321200
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Non - Mutable in CDS
MIS: 0.131 | MFS: -2.94 | #Insertions: 0
PlasmoGEM Phenotype: Essential (Pb ortholog: PBANKA_1217300)
RMgmDB ABS Phenotype: N/A
More info: PhenoPlasm Link
AlphaFill Uniprot ID: O97321
"Best" AlphaFill ligand hit: P6L ((2s)-3-{[{[(2s)-2,3-dihydroxypropyl]oxy}(hydroxy)phosphoryl]oxy}-2-[(6e)-hexadec-6-enoyloxy]propyl (8e)-octadec-8-enoate, Local RMSD=0.57) with 6FWZ (Global RMSD=4.44)
BRENDA EC Inhibitors:
| EC # | Name | EC Inhibitors |
|---|---|---|
| 2.7.8.13 | phospho-N-acetylmuramoyl-pentapeptide-transferase | EDTANH4+A-94964RU88110RU74854RU77254RU75413RU78695RU88108RU76491RU74709RU88114... |
| 2.7.8.15 | UDP-N-acetylglucosamine-dolichyl-phosphate N-acetylglucosaminephosphotransferase | UDPGTPNEMUMPPCMBDiumycinUDP-GlcNAcUDP-xyloseAmphomycinUDP-glucoseGDP-mannosetunicamycin... |
Orthology to BindingDB Entries:
| UniProt | Protein Name | Species | Homology Source | BindingDB Ligands |
|---|---|---|---|---|
| Q9H3H5 | UDP-N-acetylglucosamine--dolichyl-phosphate N-acetylglucosaminephosphotransferase | HOGENOM, OMA, OrthoDB, OrthoMCL BLAST | CHEMBL4797442CHEMBL4753142CHEMBL4752744CHEMBL4776441 | |
| O66465 | Phospho-N-acetylmuramoyl-pentapeptide-transferase | OrthoMCL BLAST | CHEMBL4286766CHEMBL4284923CHEMBL4283345CHEMBL4279378 | |
| Q03521 | Phospho-N-acetylmuramoyl-pentapeptide-transferase | Bacillus subtilis | OrthoMCL BLAST | Muraymycin D2CHEMBL1780217CHEMBL1780218CHEMBL1780219... |
| P0C1R8 | Phospho-N-acetylmuramoyl-pentapeptide-transferase | Staphylococcus aureus (strain MRSA252) | OrthoMCL BLAST | CHEMBL2048826CHEMBL2048825CHEMBL2048827CHEMBL2048828... |
Ortholog Group (OrthoMCL): OG6_101483
Most Similar Human Ortholog: A0A804HKZ3
TM-align score: 0.90 | RMSD: 1.90
Seq Identity: 0.43 | Length: 298 / 397
All Human Orthologs (OrthoMCL):
| Gene ID | Description |
|---|---|
| ENSG00000172269 | dolichyl-phosphate N-acetylglucosaminephosphotransferase 1 |
Protein Length: 397 | Molecular Weight (kDa): 46.506
UniProt ID(s): O97321
PDB ID(s): None
Isoelectric Point: 9.52
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR000715 | Glycosyl transferase, family 4 |
| InterPro | IPR033895 | UDP-GlcNAc-dolichyl-phosphate GlcNAc phosphotransferase |
| PFam | PF00953 | Glycosyl transferase, family 4 |
| Superfamily | N/A | N/A |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 5 | 23 | 13 | 47 |
| disruptive | 10 | 23 | 10 | 37 |
| missense | 10 | 23 | 10 | 37 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 7 | 51 | 23 | 37 |
| disruptive | 12 | 59 | 48 | 99 |
| missense | 12 | 51 | 39 | 66 |
PlasmoDB Total SNPs: 275
Non-coding: 253 | Synonymous: 17 | Nonsynonymous: 5 | Stop Codon: 0
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 10448855 | The complete nucleotide sequence of chromosome 3 of Plasmodium falciparum. | Bowman S, Lawson D, ..., Barrell BG | 10.1038/22964 |
| 11152613 | Predicting transmembrane protein topology with a hidden Markov model: applicationto complete genomes. | Krogh A, Larsson B, von Heijne G, Sonnhammer EL | 10.1006/jmbi.2000.4315 |
| 12368867 | Sequence of Plasmodium falciparum chromosomes 1, 3-9 and 13. | Hall N, Pain A, ..., Barrell BG | 10.1038/nature01095 |
| 11520056 | A comprehensive analysis of gene expression profiles in a yeast N-glycosylationmutant | Klebl B, Kozian D, Leberer E, Kukuruzinska MA | 10.1006/bbrc.2001.5457 |
| 15794922 | A hypomorphic allele of the first N-glycosylation gene, ALG7, causesmitochondrial defects in yeast | Mendelsohn RD, Helmerhorst EJ, Cipollo JF, Kukuruzinska MA | 10.1016/j.bbagen.2005.01.017 |
| 34465026 | Tunicamycin Potentiates Antifungal Drug Tolerance via Aneuploidy in Candida albicans | Yang F, Gritsenko V, ..., Berman J | 10.1128/mBio.02272-21 |
| 14734021 | The histidine kinases of Candida albicans: regulation of cell wall mannanbiosynthesis | Kruppa M, Jabra-Rizk MA, Meiller TF, Calderone R | 10.1016/S1567-1356(03)00201-0 |
| 27822701 | AglH, a thermophilic UDP-N-acetylglucosamine-1-phosphate:dolichyl phosphateGlcNAc-1-phosphotransferase initiating protein N-glycosylation pathway inSulfolobus acidocaldarius, is capable of complementing the eukaryal Alg7 | Meyer BH, Shams-Eldin H, Albers SV | 10.1007/s00792-016-0890-2 |
| 30185560 | Genetic screen identifies adaptive aneuploidy as a key mediator of ER stressresistance in yeast | Beaupere C, Dinatto L, ..., Labunskyy VM | 10.1073/pnas.1804264115 |
| 35222844 | Compounds targeting GPI biosynthesis or N-glycosylation are active againstPlasmodium falciparum | Fenollar A, Ros-Lucas A, ..., Izquierdo L | 10.1016/j.csbj.2022.01.029 |
| 9451016 | Cloning and functional expression of the human GlcNAc-1-P transferase, the enzymefor the committed step of the dolichol cycle, by heterologous complementation inSaccharomyces cerevisiae | Eckert V, Blank M, ..., Schwarz RT | 10.1093/glycob/8.1.77 |
| 11003550 | Chromosomal promoter replacement in Saccharomyces cerevisiae: construction ofconditional lethal strains for the cloning of glycosyltransferases from variousorganisms | Mazhari-Tabrizi R, Blank M, ..., Eckert V | 10.1023/a:1007132907235 |
| 18178736 | Identification of the archaeal alg7 gene homolog (encodingN-acetylglucosamine-1-phosphate transferase) of the N-linked glycosylation systemby cross-domain complementation in Saccharomyces cerevisiae | Shams-Eldin H, Chaban B, ..., Jarrell KF | 10.1128/JB.01778-07 |
| 22061998 | Alg14 organizes the formation of a multiglycosyltransferase complex involved ininitiation of lipid-linked oligosaccharide biosynthesis | Lu J, Takahashi T, ..., Gao XD | 10.1093/glycob/cwr162 |