PF3D7_0321500

Genome location: Pf3D7_03_v3:896,241..900,388(-)

Genome classification: Core

Function and Localization

Product Description: peptidase, putative

SignalP Peptide: N/A

# Transmembrane Domains: 0

EC Numbers: 3.4.-.- (Acting on peptide bonds (peptidases).);3.4.21.26 (Prolyl oligopeptidase)

Curated GO (PlasmoDB):

Type	GO Term	Name
Component	GO:0005634	nucleus
Function	GO:0008233	peptidase activity
Function	GO:0004252	serine-type endopeptidase activity

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	expressed	N/A	N/A
Ring	expressed	0.08	0.96
Trophozoite	expressed	0.54	0.69
Schizont	expressed	0.39	0.76
Gametocyte	expressed	0.33	0.80

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_0321500

Resistome Missense Mutations: L1038V

Resistome Compounds with Missense Mutations: MMV407834

Resistome # Samples with Disruptive Mutations: 2 (2 missense, 2 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Mutable in CDS

MIS: 1 | MFS: 0.011 | #Insertions: 4

PlasmoGEM Phenotype: N/A

RMgmDB ABS Phenotype: N/A

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: O97289

"Best" AlphaFill ligand hit: No AlphaFill hits

BRENDA EC Inhibitors:

EC #	Name	EC Inhibitors
3.4.-.-	Ex: cytosol alanyl aminopeptidase	DTTNEMKCNPheLeuTyrMetEDTAPMSFEGTACoSO4azide...
3.4.21.26	prolyl oligopeptidase	BRENDA SOAP query unsuccessful

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_102438

Most Similar Human Ortholog: C9JLK2

TM-align score: 0.70 | RMSD: 3.36

Seq Identity: 0.16 | Length: 197 / 1074

All Human Orthologs (OrthoMCL):

Gene ID	Description
ENSG00000164062	acylaminoacyl-peptide hydrolase

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	13	82	21	62
disruptive	30	109	80	161
missense	20	90	70	134

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	32	117	40	77
disruptive	58	244	129	261
missense	35	188	109	198

PlasmoDB Total SNPs: 295

Non-coding: 171 | Synonymous: 64 | Nonsynonymous: 59 | Stop Codon: 1

Protein Information

Protein Length: 1074 | Molecular Weight (kDa): 125.894

UniProt ID(s): O97289

PDB ID(s): None

Isoelectric Point: 6.57

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR001375	Peptidase S9, prolyl oligopeptidase, catalytic domain
InterPro	IPR029058	Alpha/Beta hydrolase fold
PFam	PF00326	Peptidase S9, prolyl oligopeptidase, catalytic domain
Superfamily	SSF53474	Alpha/Beta hydrolase fold

PMID	Title	Authors	DOI/Link
10448855	The complete nucleotide sequence of chromosome 3 of Plasmodium falciparum.	Bowman S, Lawson D, ..., Barrell BG	10.1038/22964
12368867	Sequence of Plasmodium falciparum chromosomes 1, 3-9 and 13.	Hall N, Pain A, ..., Barrell BG	10.1038/nature01095
23181666	Organellar proteomics reveals hundreds of novel nuclear proteins in the malariaparasite Plasmodium falciparum.	Oehring SC, Woodcroft BJ, ..., Voss TS	10.1186/gb-2012-13-11-r108