PF3D7_0321600 (DDX42)

Genome location: Pf3D7_03_v3:900,508..903,759(+)

Genome classification: Core

Function and Localization

Product Description: ATP-dependent RNA helicase DDX42, putative

SignalP Peptide: N/A

# Transmembrane Domains: 0

EC Numbers: 3.6.4.13 (RNA helicase)

Curated GO (PlasmoDB):

Type	GO Term	Name
Component	GO:0005737	cytoplasm
Component	GO:0005634	nucleus
Component	GO:1990904	ribonucleoprotein complex
Function	GO:0003723	RNA binding
Function	GO:0003724	RNA helicase activity

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	not expressed	N/A	N/A
Ring	expressed	0.20	0.91
Trophozoite	expressed	1.12	0.44
Schizont	expressed	0.93	0.51
Gametocyte	expressed	1.08	0.45

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_0321600

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Mutable in CDS

MIS: 1 | MFS: 0.124 | #Insertions: 4

PlasmoGEM Phenotype: Dispensable (Pb ortholog: PBANKA_1216900)

Relative Growth Rate: 0.98 ± 0.09
Confidence: 6.10

RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PBANKA_1216900)

Modification: Disrupted | RMgm-3256

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: O97290

"Best" AlphaFill ligand hit: CXS (3-cyclohexyl-1-propylsulfonic acid, Local RMSD=0.15) with 2WAX (Global RMSD=5.42)

BRENDA EC Inhibitors:

EC #	Name	EC Inhibitors
3.6.4.13	RNA helicase	ATPADPUDPAMPGTPGDPUTPKClNEMCDPITPTDP...

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_171471

No human ortholog(s)

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	6	33	22	52
disruptive	13	79	53	140
missense	11	71	48	128

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	12	62	42	58
disruptive	36	172	102	222
missense	24	149	91	161

PlasmoDB Total SNPs: 166

Non-coding: 119 | Synonymous: 32 | Nonsynonymous: 15 | Stop Codon: 0

Protein Information

Protein Length: 735 | Molecular Weight (kDa): 86.646

UniProt ID(s): O97290

PDB ID(s): None

Isoelectric Point: 5.49

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR001650	Helicase, C-terminal
InterPro	IPR011545	DEAD/DEAH box helicase domain
InterPro	IPR014001	Helicase superfamily 1/2, ATP-binding domain
InterPro	IPR027417	P-loop containing nucleoside triphosphate hydrolase
PFam	PF00270	DEAD/DEAH box helicase domain
PFam	PF00271	Helicase, C-terminal
Superfamily	SSF52540	P-loop containing nucleoside triphosphate hydrolase

PMID	Title	Authors	DOI/Link
10448855	The complete nucleotide sequence of chromosome 3 of Plasmodium falciparum.	Bowman S, Lawson D, ..., Barrell BG	10.1038/22964
12368867	Sequence of Plasmodium falciparum chromosomes 1, 3-9 and 13.	Hall N, Pain A, ..., Barrell BG	10.1038/nature01095
16267556	A protein interaction network of the malaria parasite Plasmodium falciparum.	LaCount DJ, Vignali M, ..., Hughes RE	10.1038/nature04104