About Gene List

PF3D7_0322100 (Prt1)

Genome location: Pf3D7_03_v3:930,302..933,014(-)

Genome classification: Core

Function and Localization

Product Description: mRNA-capping enzyme subunit beta

SignalP Peptide: N/A

# Transmembrane Domains: 0

EC Numbers: 3.1.3.33 (Polynucleotide 5'-phosphatase)

Curated GO (PlasmoDB):

Type GO Term Name
Function GO:0016887 ATP hydrolysis activity

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage LR class MCA mean MCA prop. zeros
Sporozoite not expressed N/A N/A
Ring expressed 0.21 0.89
Trophozoite expressed 0.32 0.80
Schizont expressed 0.07 0.95
Gametocyte possibly expressed 0.17 0.89

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_0322100

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Non - Mutable in CDS

MIS: 0.901 | MFS: -2.393 | #Insertions: 0

PlasmoGEM Phenotype: N/A

RMgmDB ABS Phenotype: N/A

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: C0H484

"Best" AlphaFill ligand hit: No AlphaFill hits

BRENDA EC Inhibitors:

EC # Name EC Inhibitors
3.1.3.33 polynucleotide 5'-phosphatase p-hydroxymercuribenzoate

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_130919

No human ortholog(s)

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type common rare doubleton singleton
synonymous 6 33 17 28
disruptive 31 98 27 101
missense 18 74 20 88

Any inclusion in genotype call

variant type common rare doubleton singleton
synonymous 16 55 23 55
disruptive 56 160 76 157
missense 33 118 59 100

PlasmoDB Total SNPs: 156

Non-coding: 85 | Synonymous: 39 | Nonsynonymous: 32 | Stop Codon: 0

Protein Information

Protein Length: 591 | Molecular Weight (kDa): 69.664

UniProt ID(s): C0H484

PDB ID(s): None

Isoelectric Point: 6.28

Protein Domain Annotations:

Source Family ID Description
InterPro IPR004206 mRNA triphosphatase Cet1-like
InterPro IPR033469 CYTH-like domain superfamily
InterPro IPR040343 RNA 5'-triphosphatase Cet1/Ctl1
PFam N/A N/A
Superfamily SSF55154 CYTH-like domain superfamily

Associated Publications

PMID Title Authors DOI/Link
10448855 The complete nucleotide sequence of chromosome 3 of Plasmodium falciparum. Bowman S, Lawson D, ..., Barrell BG 10.1038/22964
11248030 A yeast-like mRNA capping apparatus in Plasmodium falciparum. Ho CK, Shuman S 10.1073/pnas.061636198
12368867 Sequence of Plasmodium falciparum chromosomes 1, 3-9 and 13. Hall N, Pain A, ..., Barrell BG 10.1038/nature01095
16267556 A protein interaction network of the malaria parasite Plasmodium falciparum. LaCount DJ, Vignali M, ..., Hughes RE 10.1038/nature04104
34298922 Reprogramming mRNA Expression in Response to Defect in RNA Polymerase IIIAssembly in the Yeast Saccharomyces cerevisiae Rudzinska I, Ciesla M, ..., Boguta M 10.3390/ijms22147298