Product Description: diacylglycerol O-acyltransferase
SignalP Peptide: N/A
# Transmembrane Domains: 9
EC Numbers: 2.3.1.20 (Diacylglycerol O-acyltransferase)
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0005783 | endoplasmic reticulum |
| Component | GO:0005789 | endoplasmic reticulum membrane |
| Function | GO:0008374 | O-acyltransferase activity |
| Function | GO:0004144 | diacylglycerol O-acyltransferase activity |
| Process | GO:0019432 | triglyceride biosynthetic process |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | expressed | N/A | N/A |
| Ring | expressed | 0.09 | 0.96 |
| Trophozoite | expressed | 0.97 | 0.53 |
| Schizont | expressed | 0.61 | 0.68 |
| Gametocyte | expressed | 0.26 | 0.85 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_0322300
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Non - Mutable in CDS
MIS: 0.199 | MFS: -2.812 | #Insertions: 0
PlasmoGEM Phenotype: Essential (Pb ortholog: PBANKA_1216200)
RMgmDB ABS Phenotype: N/A
More info: PhenoPlasm Link
AlphaFill Uniprot ID: O97295
"Best" AlphaFill ligand hit: No AlphaFill hits
BRENDA EC Inhibitors:
| EC # | Name | EC Inhibitors |
|---|---|---|
| 2.3.1.20 | diacylglycerol O-acyltransferase | ATPCoAKClDTTNaFEDTAHgCl2MgCl2CaCl2MnCl2ZnSO4CHAPS... |
Orthology to BindingDB Entries:
| UniProt | Protein Name | Species | Homology Source | BindingDB Ligands |
|---|---|---|---|---|
| Q9Z2A7 | Diacylglycerol O-acyltransferase 1 | Mus musculus | OrthoDB, OrthoMCL BLAST | Urea-based Analogue, 4aUrea-based Analogue, 4bUrea-based Analogue, 4cUrea-based Analogue, 4d... |
| Q60457 | Sterol O-acyltransferase 1 | OrthoDB | CHEMBL33780 | |
| Q9ERM3 | Diacylglycerol O-acyltransferase 1 | Rattus norvegicus | OrthoDB, OrthoMCL BLAST | pyrimido[4,5-b][1,4]oxazine derivative, 2bicyclo[2.2.2]octane-pyrimido[4,5-b][1,4]oxazine deriv., 4CHEMBL2178953CHEMBL1766813... |
| P35610 | Sterol O-acyltransferase 1 | Homo sapiens | OrthoDB | bicyclo[2.2.2]octane-pyrimido[4,5-b][1,4]oxazine deriv., 4US9149492, K-604CHEMBL516093CHEMBL409855... |
| Q7TQM4 | Sterol O-acyltransferase 2 | Rattus norvegicus | OrthoDB | CHEMBL1241348Lecimibide |
| O75907 | Diacylglycerol O-acyltransferase 1 | OrthoDB, OrthoMCL BLAST | Urea-based Analogue, 4aUrea-based Analogue, 4bUrea-based Analogue, 4cUrea-based Analogue, 4d... | |
| O70536 | Sterol O-acyltransferase 1 | Rattus norvegicus | OrthoDB | CHEMBL33780LecimibideCHEMBL295079CHEMBL45964... |
| O75908 | Sterol O-acyltransferase 2 | OrthoDB | US9149492, Eflucimibe (F12511)US9149492, Compound 1AUS9149492, Compound 1BUS9149492, Compound 1C... | |
| Q61263 | Sterol O-acyltransferase 1 | Mus musculus | OrthoDB | CHEMBL416816CHEMBL300518CHEMBL44610Lecimibide... |
| A0A8I3N393 | Diacylglycerol O-acyltransferase 1 [148-636] | Canis familiaris | OrthoMCL BLAST | CHEMBL2178953 |
Ortholog Group (OrthoMCL): OG6_101524
Most Similar Human Ortholog: O75907
TM-align score: 0.75 | RMSD: 3.39
Seq Identity: 0.28 | Length: 403 / 653
All Human Orthologs (OrthoMCL):
| Gene ID | Description |
|---|---|
| ENSG00000185000 | diacylglycerol O-acyltransferase 1 |
| ENSG00000285482 | diacylglycerol O-acyltransferase 1 |
Protein Length: 653 | Molecular Weight (kDa): 78.114
PDB ID(s): None
Isoelectric Point: 8.99
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR004299 | Membrane bound O-acyl transferase, MBOAT |
| InterPro | IPR014371 | Sterol O-acyltransferase, ACAT/DAG/ARE types |
| InterPro | IPR027251 | Diacylglycerol O-acyltransferase 1 |
| PFam | PF03062 | Membrane bound O-acyl transferase, MBOAT |
| Superfamily | N/A | N/A |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 5 | 29 | 14 | 52 |
| disruptive | 15 | 63 | 33 | 76 |
| missense | 11 | 41 | 23 | 49 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 11 | 77 | 32 | 43 |
| disruptive | 37 | 150 | 74 | 193 |
| missense | 19 | 98 | 52 | 114 |
PlasmoDB Total SNPs: 285
Non-coding: 204 | Synonymous: 62 | Nonsynonymous: 18 | Stop Codon: 1
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 10448855 | The complete nucleotide sequence of chromosome 3 of Plasmodium falciparum. | Bowman S, Lawson D, ..., Barrell BG | 10.1038/22964 |
| 12368867 | Sequence of Plasmodium falciparum chromosomes 1, 3-9 and 13. | Hall N, Pain A, ..., Barrell BG | 10.1038/nature01095 |
| 15110461 | Neutral lipid synthesis and storage in the intraerythrocytic stages of Plasmodiumfalciparum. | Vielemeyer O, McIntosh MT, Joiner KA, Coppens I | 10.1016/j.molbiopara.2003.08.017 |
| 38296006 | Selenium yeast improve growth, serum biochemical indices, metabolic ability,antioxidant capacity and immunity in black carp Mylopharyngodnpiceus | Zhang P, Zhang C, ..., Mi H | 10.1016/j.fsi.2024.109414 |