Product Description: Rh5 interacting protein
SignalP Peptide: MFRIFFTLLIIILIKKTSA
# Transmembrane Domains: 0
EC Numbers: None
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0033644 | host cell membrane |
| Component | GO:0043655 | host extracellular space |
| Component | GO:0016020 | membrane |
| Component | GO:0020009 | microneme |
| Component | GO:0034494 | microneme lumen |
| Component | GO:0032991 | protein-containing complex |
| Function | GO:0046812 | host cell surface binding |
| Function | GO:0005515 | protein binding |
| Process | GO:0044409 | entry into host |
| Process | GO:0051205 | protein insertion into membrane |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | possibly expressed | N/A | N/A |
| Ring | not expressed | 0.71 | 0.78 |
| Trophozoite | not expressed | 0.08 | 0.96 |
| Schizont | expressed | 0.76 | 0.75 |
| Gametocyte | possibly expressed | 0.54 | 0.73 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_0323400
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Mutable in CDS
MIS: 0.988 | MFS: -2.702 | #Insertions: 1
PlasmoGEM Phenotype: Essential (Pb ortholog: PBANKA_1215100)
RMgmDB ABS Phenotype: N/A
More info: PhenoPlasm Link
AlphaFill Uniprot ID: O97302
"Best" AlphaFill ligand hit: TMA (tetramethylammonium ion, Local RMSD=0.12) with 4YLQ (Global RMSD=10.21)
No associated EC numbersNo evidence of orthology to BindingDB entries
Ortholog Group (OrthoMCL): OG6_100900
Most Similar Human Ortholog: H0YN80
TM-align score: 0.40 | RMSD: 2.93
Seq Identity: 0.00 | Length: 33 / 1086
All Human Orthologs (OrthoMCL):
| Gene ID | Description |
|---|---|
| ENSG00000138829 | fibrillin 2 |
| ENSG00000142449 | fibrillin 3 |
| ENSG00000166147 | fibrillin 1 |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 2 | 31 | 18 | 40 |
| disruptive | 33 | 46 | 31 | 87 |
| missense | 31 | 46 | 31 | 82 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 5 | 49 | 26 | 61 |
| disruptive | 50 | 109 | 63 | 150 |
| missense | 44 | 94 | 57 | 113 |
PlasmoDB Total SNPs: 130
Non-coding: 68 | Synonymous: 38 | Nonsynonymous: 24 | Stop Codon: 0
Protein Length: 1086 | Molecular Weight (kDa): 125.893
UniProt ID(s): A0A193PDI9, A0A193PDK3, A0A193PDK8, A0A193PDL3, A0A193PDL9, A0A193PDP4, A0A193PDQ8, A0A193PE01, A0A193PE05, A0A193PE07, O97302
Isoelectric Point: 6.73
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR000742 | EGF-like domain |
| PFam | N/A | N/A |
| Superfamily | N/A | N/A |
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 10448855 | The complete nucleotide sequence of chromosome 3 of Plasmodium falciparum. | Bowman S, Lawson D, ..., Barrell BG | 10.1038/22964 |
| 12368867 | Sequence of Plasmodium falciparum chromosomes 1, 3-9 and 13. | Hall N, Pain A, ..., Barrell BG | 10.1038/nature01095 |
| 16267556 | A protein interaction network of the malaria parasite Plasmodium falciparum. | LaCount DJ, Vignali M, ..., Hughes RE | 10.1038/nature04104 |
| 21909261 | An EGF-like protein forms a complex with PfRh5 and is required for invasion ofhuman erythrocytes by Plasmodium falciparum. | Chen L, Lopaticki S, ..., Cowman AF | 10.1371/journal.ppat.1002199 |
| 25583518 | Multiprotein complex between the GPI-anchored CyRPA with PfRH5 and PfRipr iscrucial for Plasmodium falciparum erythrocyte invasion. | Reddy KS, Amlabu E, ..., Gaur D | https://pubmed.ncbi.nlm.nih.gov/25583518/ |
| 27692771 | Identification of Plasmodium falciparum reticulocyte binding protein homologue5-interacting protein, PfRipr, as a highly conserved blood-stage malaria vaccinecandidate. | Ntege EH, Arisue N, ..., Tsuboi T | 10.1016/j.vaccine.2016.09.028 |
| 30542156 | Structure of Plasmodium falciparum Rh5-CyRPA-Ripr invasion complex. | Wong W, Huang R, ..., Cowman AF | 10.1038/s41586-018-0779-6 |
| 32313230 | Antibodies against a short region of PfRipr inhibit Plasmodium falciparummerozoite invasion and PfRipr interaction with Rh5 and SEMA7A. | Nagaoka H, Kanoi BN, ..., Takashima E | 10.1038/s41598-020-63611-6 |
| 32900802 | The Structure of the Cysteine-Rich Domain of Plasmodium falciparum P113Identifies the Location of the RH5 Binding Site | Campeotto I, Galaway F, ..., Wright GJ | 10.1128/mBio.01566-20 |
| 34162366 | The Plasmodium falciparum Rh5 invasion protein complex reveals an excess of rarevariant mutations | Ndwiga L, Osoti V, ..., Ochola-Oyier LI | 10.1186/s12936-021-03815-x |
| 32678144 | Bliss' and Loewe's additive and synergistic effects in Plasmodium falciparumgrowth inhibition by AMA1-RON2L, RH5, RIPR and CyRPA antibody combinations | Azasi Y, Gallagher SK, ..., Miura K | 10.1038/s41598-020-67877-8 |
| 34305923 | Identification and Immune Assessment of T Cell Epitopes in Five Plasmodiumfalciparum Blood Stage Antigens to Facilitate Vaccine Candidate Selection andOptimization | Kotraiah V, Phares TW, ..., Noe AR | 10.3389/fimmu.2021.690348 |
| 36211427 | Process development and preclinical evaluation of a major Plasmodium falciparumblood stage vaccine candidate, Cysteine-Rich Protective Antigen (CyRPA) | Somanathan A, Mian SY, ..., Chauhan VS | 10.3389/fimmu.2022.1005332 |
| 38123677 | The PfRCR complex bridges malaria parasite and erythrocyte during invasion | Farrell B, Alam N, ..., Higgins MK | 10.1038/s41586-023-06856-1 |
| 32359873 | The RH5-CyRPA-Ripr Complex as a Malaria Vaccine Target | Ragotte RJ, Higgins MK, Draper SJ | 10.1016/j.pt.2020.04.003 |
| 31185066 | Divergent roles for the RH5 complex components, CyRPA and RIPR in human-infectivemalaria parasites | Knuepfer E, Wright KE, ..., Holder AA | 10.1371/journal.ppat.1007809 |
| 38406638 | Limited genetic variations of the Rh5-CyRPA-Ripr invasion complex in Plasmodiumfalciparum parasite population in selected malaria-endemic regions, Kenya | Waweru H, Kanoi BN, ..., Gitaka J | 10.3389/fitd.2023.1102265 |
| 34694918 | Plasmodium falciparum Cysteine-Rich Protective Antigen (CyRPA) Elicits DetectableLevels of Invasion-Inhibitory Antibodies during Natural Infection in Humans | Mian SY, Somanathan A, ..., Gaur D | 10.1128/IAI.00377-21 |