Genome location: Pf3D7_04_v3:45,555..56,860(-)
Genome classification: SubtelomericHypervariable
Product Description: erythrocyte membrane protein 1, PfEMP1
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: None
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0020002 | host cell plasma membrane |
| Component | GO:0020030 | infected host cell surface knob |
| Function | GO:0050839 | cell adhesion molecule binding |
| Process | GO:0020033 | antigenic variation |
| Process | GO:0098609 | cell-cell adhesion |
| Process | GO:0020035 | cytoadherence to microvasculature, mediated by symbiont protein |
| Process | GO:0020013 | modulation by symbiont of host erythrocyte aggregation |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | expressed | N/A | N/A |
| Ring | expressed | 0.01 | 1.00 |
| Trophozoite | expressed | 0.00 | 1.00 |
| Schizont | expressed | 0.00 | 1.00 |
| Gametocyte | expressed | 0.01 | 1.00 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_0400400
Resistome Missense Mutations: A175T, Q445K, E449V, T1125P, V1639M, P1680S, I1709K, E1712D, E1712A, D1718N, L1720V, T2183A, T2183K, Q2184E, Q2188H, L2743I, I3157V
Resistome Compounds with Missense Mutations: AN13762, KAE678/NITD678, MMV006767, MMV007564
Resistome # Samples with Disruptive Mutations: 7 (7 missense, 7 "interesting" missense)
Zhang Phenotype: Non - Mutable in CDS *tentative
MIS: 0.977 | MFS: -2.248 | #Insertions: 0
PlasmoGEM Phenotype: N/A
RMgmDB ABS Phenotype: N/A
More info: PhenoPlasm Link
AlphaFill Uniprot ID: N/A
"Best" AlphaFill ligand hit: No AlphaFill hits
No associated EC numbersNo evidence of orthology to BindingDB entries
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
No passing variants found
PlasmoDB Total SNPs: 4315
Non-coding: 360 | Synonymous: 872 | Nonsynonymous: 3054 | Stop Codon: 29
Protein Length: 3467 | Molecular Weight (kDa): 398.219
UniProt ID(s): A0A0U1XT24, A0A191VZ00, A0A191VZ17, A0A191VZ32, A0A191VZ46, A0A191VZ60, A0A191VZ63, A0A191VZ81, A0A191VZ85, A0A191VZ86, A0A191VZ87, A0A191VZA9, A0A191VZB1, A0A191VZB3, A0A191VZC0, A0A191VZC2, A0A191VZC4, A0A191VZC8, A0A191VZE0, A0A191VZE8, A0A191VZF7, A0A5P1A089, A0A5P1A0M0, A0A5P1A0N9, A0A5P1A0P2, A0A5P1A136, A0A5P1A146, A0A5P1A178, A0A5P1A185, A0A5P1A1M5, A0A5P1A1X5, A0A5P1A235, A0A5P1A2I5, A0A5P1A2J7, A0A5P1A2P8, A0A5P1A346, A0A5P1A352, A0A5P1A3A6, A3R6S2, A3R6S4, A3R6V0, A3R6V1, A3R6V3, E0A3B2, I2CIW8, I2CIX5, I2CIX6, I2CIX7, I2CIY0, Q26032, Q6UDW5, Q8I218, Q8T326
PDB ID(s): None
Isoelectric Point: 5.63
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR004258 | Duffy-binding-like domain |
| InterPro | IPR008602 | Duffy-antigen binding |
| InterPro | IPR029210 | Plasmodium falciparum erythrocyte membrane protein-1, N-terminal segment |
| InterPro | IPR029211 | Plasmodium falciparum erythrocyte membrane protein 1, acidic terminal segment |
| InterPro | IPR041480 | Cysteine-rich interdomain region 1 gamma |
| PFam | PF03011 | Duffy-binding-like domain |
| PFam | PF05424 | Duffy-antigen binding |
| PFam | PF15445 | Plasmodium falciparum erythrocyte membrane protein 1, acidic terminal segment |
| PFam | PF15447 | Plasmodium falciparum erythrocyte membrane protein-1, N-terminal segment |
| PFam | PF18562 | Cysteine-rich interdomain region 1 gamma |
| Superfamily | SSF140924 | N/A |
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 9230440 | P. falciparum rosetting mediated by a parasite-variant erythrocyte membraneprotein and complement-receptor 1. | Rowe JA, Moulds JM, Newbold CI, Miller LH | 10.1038/40888 |
| 9291676 | Knob proteins in falciparum malaria. | Sharma YD | https://pubmed.ncbi.nlm.nih.gov/9291676/ |
| 9786187 | A recombinant peptide based on PfEMP-1 blocks and reverses adhesion ofmalaria-infected red blood cells to CD36 under flow. | Cooke BM, Nicoll CL, Baruch DI, Coppel RL | 10.1046/j.1365-2958.1998.01040.x |
| 11316367 | Variant antigens of Plasmodium falciparum encoded by the var multigenic familyare multifunctional macromolecules. | Nogueira PA, Wunderlich G, Pereira da Silva LH | 10.1016/s0923-2508(01)01184-6 |
| 11420100 | Molecules on the surface of the Plasmodium falciparum infected erythrocyte andtheir role in malaria pathogenesis and immune evasion. | Craig A, Scherf A | https://pubmed.ncbi.nlm.nih.gov/11420100/ |
| 12368867 | Sequence of Plasmodium falciparum chromosomes 1, 3-9 and 13. | Hall N, Pain A, ..., Barrell BG | 10.1038/nature01095 |
| 18785843 | Antigenic variation in Plasmodium falciparum. | Scherf A, Lopez-Rubio JJ, Riviere L | 10.1146/annurev.micro.61.080706.093134 |