About Gene List

PF3D7_0401900 (ACS6)

Genome location: Pf3D7_04_v3:108,357..111,662(-)

Genome classification: Core

Function and Localization

Product Description: acyl-CoA synthetase

SignalP Peptide: N/A

# Transmembrane Domains: 1

EC Numbers: 6.2.1.3 (Long-chain-fatty-acid--CoA ligase)

Curated GO (PlasmoDB):

Type GO Term Name
Component GO:0020036 Maurer's cleft
Component GO:0005783 endoplasmic reticulum
Component GO:0016020 membrane
Function GO:0004467 long-chain fatty acid-CoA ligase activity
Process GO:0015908 fatty acid transport

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage LR class MCA mean MCA prop. zeros
Sporozoite not expressed N/A N/A
Ring not expressed 1.44 0.45
Trophozoite expressed 0.21 0.89
Schizont possibly expressed 0.20 0.88
Gametocyte not expressed 0.09 0.95

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_0401900

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Mutable in CDS

MIS: 1 | MFS: -0.574 | #Insertions: 2

PlasmoGEM Phenotype: Slow (Pb ortholog: PBANKA_1453300)

  • Relative Growth Rate: 0.41 ± 0.29
  • Confidence: 3.84

RMgmDB ABS Phenotype: N/A

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: Q8I0V8

"Best" AlphaFill ligand hit: AMP (adenosine monophosphate, Local RMSD=0.07) with 1AMU (Global RMSD=3.13)

BRENDA EC Inhibitors:

EC # Name EC Inhibitors
6.2.1.3 long-chain-fatty-acid-CoA ligase ATPAMPNEMSDSEDTAsaltsoleateGW1929Brij 58Tween 80naproxentriacsin...

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_100152

Most Similar Human Ortholog: B7Z3Z9

TM-align score: 0.90 | RMSD: 2.47

Seq Identity: 0.23 | Length: 513 / 971

All Human Orthologs (OrthoMCL):

Gene ID Description
ENSG00000151726 acyl-CoA synthetase long chain family member 1
ENSG00000164398 acyl-CoA synthetase long chain family member 6
ENSG00000197142 acyl-CoA synthetase long chain family member 5
ENSG00000281938 novel transcript

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type common rare doubleton singleton
synonymous 10 50 27 37
disruptive 61 111 51 135
missense 42 89 44 100

Any inclusion in genotype call

variant type common rare doubleton singleton
synonymous 13 88 26 41
disruptive 90 200 95 207
missense 60 154 79 136

PlasmoDB Total SNPs: 132

Non-coding: 8 | Synonymous: 55 | Nonsynonymous: 69 | Stop Codon: 0

Protein Information

Protein Length: 971 | Molecular Weight (kDa): 110.797

UniProt ID(s): Q8I0V8

PDB ID(s): None

Isoelectric Point: 9.04

Protein Domain Annotations:

Source Family ID Description
InterPro IPR000873 AMP-dependent synthetase/ligase
InterPro IPR020845 AMP-binding, conserved site
PFam PF00501 AMP-dependent synthetase/ligase
Superfamily SSF56801 N/A

Associated Publications

PMID Title Authors DOI/Link
12368867 Sequence of Plasmodium falciparum chromosomes 1, 3-9 and 13. Hall N, Pain A, ..., Barrell BG 10.1038/nature01095
31631203 A network-based approach reveals novel invasion and Maurer's clefts-relatedproteins in Plasmodium falciparum. Das D, Krishnan SR, Roy A, Bulusu G 10.1039/c9mo00124g
30995911 Hospital-derived antibody profiles of malaria patients in Southwest India Venkatesh A, Jain A, ..., Rathod PK 10.1186/s12936-019-2771-5