PF3D7_0403500 (USP)

Genome location: Pf3D7_04_v3:195,272..196,988(+)

Genome classification: Core

Function and Localization

Product Description: ubiquitin-specific protease

SignalP Peptide: N/A

# Transmembrane Domains: 0

EC Numbers: 3.1.2.15 (Deleted entry);3.4.19.12 (Ubiquitinyl hydrolase 1)

Curated GO (PlasmoDB):

Type	GO Term	Name
Component	GO:0005783	endoplasmic reticulum
Component	GO:0000502	proteasome complex
Function	GO:0101005	deubiquitinase activity
Process	GO:0016579	protein deubiquitination

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	expressed	N/A	N/A
Ring	possibly expressed	0.12	0.94
Trophozoite	expressed	0.45	0.73
Schizont	expressed	0.22	0.85
Gametocyte	possibly expressed	0.29	0.82

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_0403500

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Non - Mutable in CDS

MIS: 0.197 | MFS: -3.366 | #Insertions: 0

PlasmoGEM Phenotype: Essential (Pb ortholog: PBANKA_1001100)

Relative Growth Rate: 0.04 ± 0.24
Confidence: 4.26

RMgmDB ABS Phenotype: N/A

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: Q8I1Z5

"Best" AlphaFill ligand hit: MIX (mitoxantrone, Local RMSD=0.01) with 7R2G (Global RMSD=4.94)

BRENDA EC Inhibitors:

EC #	Name	EC Inhibitors
3.1.2.15	ubiquitin thiolesterase	No BRENDA inhibitors
3.4.19.12	ubiquitinyl hydrolase 1	KClNEMDTTEDTAEGTARNAisiRNApeptidesAntibodyubiquitinLDN-574442-propenal...

Orthology to BindingDB Entries:

UniProt	Protein Name	Species	Homology Source	BindingDB Ligands
O75604	Ubiquitin carboxyl-terminal hydrolase 2	Homo sapiens	OrthoDB	LCACNLCAELCAHALCAE...
P40818	Ubiquitin carboxyl-terminal hydrolase 8	Homo sapiens	OrthoDB	CHEMBL2398212CHEMBL5172138CHEMBL5196686CHEMBL5173239...
Q9P275	Ubiquitin carboxyl-terminal hydrolase 36		OrthoDB	CHEMBL5172138CHEMBL5173239CHEMBL5185273
O94782	Ubiquitin carboxyl-terminal hydrolase 1		OrthoDB	9-(4-(3,5-dimethyl-1H-US10189841, Compound I-2US10399980, Compound I-3US10399980, Compound I-4...
Q70CQ3	Ubiquitin carboxyl-terminal hydrolase 30		OrthoDB	US11066365, Example 1US11390584, Example 2US11390584, Example 3US11390584, Example 4...

Orthology Information

Ortholog Group (OrthoMCL): OG6_208753

No human ortholog(s)

Protein Information

Protein Length: 400 | Molecular Weight (kDa): 47.264

UniProt ID(s): Q8I1Z5

PDB ID(s): None

Isoelectric Point: 8.92

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR001394	Peptidase C19, ubiquitin carboxyl-terminal hydrolase
InterPro	IPR038765	Papain-like cysteine peptidase superfamily
PFam	PF00443	Peptidase C19, ubiquitin carboxyl-terminal hydrolase
Superfamily	SSF54001	Papain-like cysteine peptidase superfamily

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	3	27	11	30
disruptive	10	22	17	40
missense	6	14	15	32

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	6	49	18	34
disruptive	15	54	44	85
missense	6	34	35	65

PlasmoDB Total SNPs: 85

Non-coding: 59 | Synonymous: 19 | Nonsynonymous: 7 | Stop Codon: 0