PF3D7_0403900 (SET8)

Genome location: Pf3D7_04_v3:218,611..222,971(+)

Genome classification: Core

Function and Localization

Product Description: SET domain protein, putative

SignalP Peptide: N/A

# Transmembrane Domains: 0

EC Numbers: 2.1.1.43 (Transferred entry: 2.1.1.354)

Curated GO (PlasmoDB): None

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	not expressed	N/A	N/A
Ring	not expressed	0.19	0.91
Trophozoite	possibly expressed	0.33	0.80
Schizont	expressed	0.87	0.54
Gametocyte	expressed	1.11	0.47

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_0403900

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Mutable in CDS

MIS: 1 | MFS: -1.974 | #Insertions: 3

PlasmoGEM Phenotype: Dispensable (Pb ortholog: PBANKA_1202900)

Relative Growth Rate: 0.94 ± 0.19
Confidence: 4.73

RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PBANKA_1001600)

Modification: Disrupted | RMgm-2704

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: Q8I1Z0

"Best" AlphaFill ligand hit: SAM (s-adenosylmethionine, Local RMSD=0.19) with 4IJ8 (Global RMSD=3.00)

BRENDA EC Inhibitors:

EC #	Name	EC Inhibitors
2.1.1.43	histone-lysine N-methyltransferase	BRENDA SOAP query unsuccessful

Orthology to BindingDB Entries:

UniProt	Protein Name	Species	Homology Source	BindingDB Ligands
Q9NQR1	N-lysine methyltransferase KMT5A		OrthoDB, OrthoMCL BLAST	CHEMBL3318284CHEMBL3318285CHEMBL3318285CHEMBL3318289...

Orthology Information

Ortholog Group (OrthoMCL): OG6_105827

Most Similar Human Ortholog: Q9NQR1

TM-align score: 0.44 | RMSD: 4.28

Seq Identity: 0.33 | Length: 206 / 1186

All Human Orthologs (OrthoMCL):

Gene ID	Description
ENSG00000183955	lysine methyltransferase 5A

Protein Information

Protein Length: 1186 | Molecular Weight (kDa): 142.738

UniProt ID(s): Q8I1Z0

PDB ID(s): None

Isoelectric Point: 8.55

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR001214	SET domain
PFam	PF00856	SET domain
Superfamily	SSF82199	N/A

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	19	55	34	81
disruptive	52	204	109	218
missense	34	162	92	180

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	30	113	58	85
disruptive	113	371	150	371
missense	69	290	127	239

PlasmoDB Total SNPs: 223

Non-coding: 76 | Synonymous: 75 | Nonsynonymous: 72 | Stop Codon: 0