PF3D7_0404900 (P41)

Genome location: Pf3D7_04_v3:266,566..268,913(-)

Genome classification: Core

Function and Localization

Product Description: 6-cysteine protein P41

SignalP Peptide: MKGVIFCLVVLLWRQAWVSS

# Transmembrane Domains: 0

EC Numbers: None

Curated GO (PlasmoDB):

Type	GO Term	Name
Component	GO:0046658	anchored component of plasma membrane
Component	GO:0009986	cell surface
Component	GO:0020004	symbiont-containing vacuolar space
Function	GO:0005515	protein binding
Process	GO:0044409	entry into host

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	expressed	N/A	N/A
Ring	expressed	0.05	0.97
Trophozoite	expressed	0.16	0.90
Schizont	expressed	0.93	0.49
Gametocyte	expressed	0.05	0.97

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_0404900

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Mutable in CDS

MIS: 0.799 | MFS: -3.133 | #Insertions: 1

PlasmoGEM Phenotype: Dispensable (Pb ortholog: PBANKA_1107600)

Relative Growth Rate: 0.98 ± 0.06
Confidence: 7.03

RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PBANKA_1002600)

Modification: Disrupted | RMgm-2711

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: Q8I1Y0

"Best" AlphaFill ligand hit: No AlphaFill hits

No associated EC numbers

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_160720

No human ortholog(s)

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	4	17	13	25
disruptive	10	34	25	76
missense	10	32	25	67

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	6	39	13	25
disruptive	13	90	45	117
missense	13	81	39	79

PlasmoDB Total SNPs: 174

Non-coding: 155 | Synonymous: 6 | Nonsynonymous: 12 | Stop Codon: 1

Protein Information

Protein Length: 378 | Molecular Weight (kDa): 43.088

UniProt ID(s): Q8I1Y0

PDB ID(s): 4YS4, 7S7Q

Isoelectric Point: 8.4

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR010884	6-Cysteine (6-Cys) domain
PFam	PF07422	6-Cysteine (6-Cys) domain
Superfamily	N/A	N/A

Associated Publications

PMID	Title	Authors	DOI/Link
12368867	Sequence of Plasmodium falciparum chromosomes 1, 3-9 and 13.	Hall N, Pain A, ..., Barrell BG	10.1038/nature01095
16203726	Distinct protein classes including novel merozoite surface antigens in Raft-likemembranes of Plasmodium falciparum.	Sanders PR, Gilson PR, ..., Crabb BS	10.1074/jbc.M509631200
16267556	A protein interaction network of the malaria parasite Plasmodium falciparum.	LaCount DJ, Vignali M, ..., Hughes RE	10.1038/nature04104
22848665	Biochemical and functional analysis of two Plasmodium falciparum blood-stage6-cys proteins: P12 and P41.	Taechalertpaisarn T, Crosnier C, ..., Crabb BS	10.1371/journal.pone.0041937
23511632	Structural and biochemical characterization of Plasmodium falciparum 12 (Pf12)reveals a unique interdomain organization and the potential for an antiparallelarrangement with Pf41.	Tonkin ML, Arredondo SA, ..., Boulanger MJ	10.1074/jbc.M113.455667
28202027	A novel Pfs38 protein complex on the surface of Plasmodium falciparum blood-stagemerozoites.	Paul G, Deshmukh A, ..., Malhotra P	10.1186/s12936-017-1716-0