PF3D7_0405000 (DDX51)

Genome location: Pf3D7_04_v3:268,919..271,537(-)

Genome classification: Core

Function and Localization

Product Description: ATP-dependent RNA helicase DDX51, putative

SignalP Peptide: N/A

# Transmembrane Domains: 0

EC Numbers: 3.6.4.13 (RNA helicase)

Curated GO (PlasmoDB):

Type	GO Term	Name
Component	GO:0005634	nucleus
Function	GO:0003724	RNA helicase activity
Process	GO:0016070	RNA metabolic process

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	possibly expressed	N/A	N/A
Ring	expressed	0.35	0.82
Trophozoite	expressed	0.50	0.71
Schizont	expressed	0.32	0.80
Gametocyte	expressed	0.43	0.75

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_0405000

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Non - Mutable in CDS

MIS: 0.131 | MFS: -3.135 | #Insertions: 0

PlasmoGEM Phenotype: Essential (Pb ortholog: PBANKA_1002700)

Relative Growth Rate: 0.07 ± 0.15
Confidence: 5.15

RMgmDB ABS Phenotype: N/A

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: Q8I1X9

"Best" AlphaFill ligand hit: CXS (3-cyclohexyl-1-propylsulfonic acid, Local RMSD=0.09) with 2WAX (Global RMSD=4.39)

BRENDA EC Inhibitors:

EC #	Name	EC Inhibitors
3.6.4.13	RNA helicase	ATPADPUDPAMPGTPGDPUTPKClNEMCDPITPTDP...

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_103754

Most Similar Human Ortholog: Q8N8A6

TM-align score: 0.71 | RMSD: 3.14

Seq Identity: 0.21 | Length: 444 / 567

All Human Orthologs (OrthoMCL):

Gene ID	Description
ENSG00000185163	DEAD-box helicase 51
ENSG00000288503	DEAD-box helicase 51

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	3	29	15	39
disruptive	9	38	47	85
missense	7	38	45	75

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	7	65	30	48
disruptive	17	125	76	184
missense	12	105	61	113

PlasmoDB Total SNPs: 112

Non-coding: 84 | Synonymous: 20 | Nonsynonymous: 8 | Stop Codon: 0

Protein Information

Protein Length: 567 | Molecular Weight (kDa): 67.689

UniProt ID(s): Q8I1X9

PDB ID(s): None

Isoelectric Point: 9.29

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR001650	Helicase, C-terminal
InterPro	IPR011545	DEAD/DEAH box helicase domain
InterPro	IPR014001	Helicase superfamily 1/2, ATP-binding domain
InterPro	IPR027417	P-loop containing nucleoside triphosphate hydrolase
PFam	PF00270	DEAD/DEAH box helicase domain
PFam	PF00271	Helicase, C-terminal
Superfamily	SSF52540	P-loop containing nucleoside triphosphate hydrolase