Product Description: liver specific protein 2, putative
SignalP Peptide: N/A
# Transmembrane Domains: 2
EC Numbers: None
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0030430 | host cell cytoplasm |
| Component | GO:0042025 | host cell nucleus |
| Component | GO:0005634 | nucleus |
| Component | GO:0020003 | symbiont-containing vacuole |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | not expressed | N/A | N/A |
| Ring | possibly expressed | 0.13 | 0.94 |
| Trophozoite | possibly expressed | 0.09 | 0.95 |
| Schizont | possibly expressed | 0.12 | 0.92 |
| Gametocyte | expressed | 0.17 | 0.90 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_0405300
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Mutable in CDS
MIS: 1 | MFS: -0.408 | #Insertions: 6
PlasmoGEM Phenotype: N/A
RMgmDB ABS Phenotype: Not tested (Pb ortholog: PBANKA_1003000)
Modification: Tagged | RMgm-5193
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8I1X6
"Best" AlphaFill ligand hit: AN2 (amp phosphoramidate, Local RMSD=3.77) with 3IED (Global RMSD=18.87)
No associated EC numbersNo evidence of orthology to BindingDB entries
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 44 | 70 | 39 | 98 |
| disruptive | 314 | 368 | 140 | 320 |
| missense | 200 | 248 | 108 | 231 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 57 | 119 | 75 | 107 |
| disruptive | 432 | 554 | 185 | 381 |
| missense | 265 | 383 | 140 | 263 |
PlasmoDB Total SNPs: 466
Non-coding: 0 | Synonymous: 189 | Nonsynonymous: 276 | Stop Codon: 1
Protein Length: 1964 | Molecular Weight (kDa): 233.714
PDB ID(s): None
Isoelectric Point: 5.38
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR001451 | Hexapeptide repeat |
| InterPro | IPR010884 | 6-Cysteine (6-Cys) domain |
| InterPro | IPR011004 | Trimeric LpxA-like superfamily |
| PFam | PF00132 | Hexapeptide repeat |
| PFam | PF07422 | 6-Cysteine (6-Cys) domain |
| Superfamily | SSF51161 | Trimeric LpxA-like superfamily |
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 12368867 | Sequence of Plasmodium falciparum chromosomes 1, 3-9 and 13. | Hall N, Pain A, ..., Barrell BG | 10.1038/nature01095 |
| 23216750 | Liver-specific protein 2: a Plasmodium protein exported to the hepatocytecytoplasm and required for merozoite formation. | Orito Y, Ishino T, ..., Yuda M | 10.1111/mmi.12083 |
| 30379851 | Identification of Plasmodium falciparum nuclear proteins by mass spectrometry andproposed protein annotation. | Briquet S, Ourimi A, ..., Vaquero C | 10.1371/journal.pone.0205596 |
| 33228734 | Definition of constitutive and stage-enriched promoters in the rodent malariaparasite, Plasmodium yoelii | Bowman LM, Finger LE, Hart KJ, Lindner SE | 10.1186/s12936-020-03498-w |
| 31094679 | The Plasmodium liver-specific protein 2 (LISP2) is an early marker of liver stagedevelopment | Gupta DK, Dembele L, ..., Diagana TT | 10.7554/eLife.43362 |
| 27241521 | Protective efficacy and safety of liver stage attenuated malaria parasites | Kumar H, Sattler JM, ..., Frischknecht F | 10.1038/srep26824 |
| 29440367 | A Plasmodium Parasite with Complete Late Liver Stage Arrest Protects againstPreerythrocytic and Erythrocytic Stage Infection in Mice | Vaughan AM, Sack BK, ..., Kappe SHI | 10.1128/IAI.00088-18 |
| 36445080 | The PTEX Pore Component EXP2 Is Important for Intrahepatic Development during thePlasmodium Liver Stage | Hussain T, Linera-Gonzalez J, ..., Beck JR | 10.1128/mbio.03096-22 |
| 24691399 | D-Glucose concentration is the key factor facilitating liver stage maturation ofPlasmodium | Itani S, Torii M, Ishino T | 10.1016/j.parint.2014.03.004 |
| 32267675 | Dual-Luciferase-Based Fast and Sensitive Detection of Malaria Hypnozoites for theDiscovery of Antirelapse Compounds | Voorberg-van der Wel AM, Zeeman AM, ..., Kocken CHM | 10.1021/acs.analchem.0c00547 |
| 25874388 | In vivo and in vitro characterization of a Plasmodium liver stage-specificpromoter | De Niz M, Helm S, ..., Heussler VT | 10.1371/journal.pone.0123473 |
| 26348884 | In silico identification of genetically attenuated vaccine candidate genes forPlasmodium liver stage | Kumar H, Frischknecht F, Mair GR, Gomes J | 10.1016/j.meegid.2015.09.002 |