Product Description: pre-mRNA-processing-splicing factor 8, putative
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: None
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0005682 | U5 snRNP |
| Component | GO:0020011 | apicoplast |
| Component | GO:0071013 | catalytic step 2 spliceosome |
| Component | GO:0005634 | nucleus |
| Component | GO:0005681 | spliceosomal complex |
| Function | GO:0030619 | U1 snRNA binding |
| Function | GO:0030620 | U2 snRNA binding |
| Function | GO:0030623 | U5 snRNA binding |
| Function | GO:0017070 | U6 snRNA binding |
| Function | GO:0097157 | pre-mRNA intronic binding |
| Process | GO:0008380 | RNA splicing |
| Process | GO:0000375 | RNA splicing, via transesterification reactions |
| Process | GO:0000244 | spliceosomal tri-snRNP complex assembly |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | expressed | N/A | N/A |
| Ring | expressed | 1.52 | 0.38 |
| Trophozoite | expressed | 1.73 | 0.24 |
| Schizont | expressed | 0.87 | 0.54 |
| Gametocyte | expressed | 1.22 | 0.41 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_0405400
Resistome Missense Mutations: L1958F
Resistome Compounds with Missense Mutations: MMV982556
Resistome # Samples with Disruptive Mutations: 2 (2 missense, 2 "interesting" missense)
Zhang Phenotype: Non - Mutable in CDS
MIS: 0.17 | MFS: -2.781 | #Insertions: 0
PlasmoGEM Phenotype: Essential (Pb ortholog: PBANKA_1003100)
RMgmDB ABS Phenotype: N/A
More info: PhenoPlasm Link
AlphaFill Uniprot ID: N/A
"Best" AlphaFill ligand hit: No AlphaFill hits
No associated EC numbersNo evidence of orthology to BindingDB entries
Ortholog Group (OrthoMCL): OG6_101765
Most Similar Human Ortholog: Not available
TM-align score: 0.00 | RMSD: 0.00
Seq Identity: 0.00 | Length: / 3136
All Human Orthologs (OrthoMCL):
| Gene ID | Description |
|---|---|
| ENSG00000174231 | pre-mRNA processing factor 8 |
| ENSG00000274442 | pre-mRNA processing factor 8 |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 30 | 177 | 85 | 191 |
| disruptive | 63 | 229 | 132 | 291 |
| missense | 45 | 191 | 116 | 255 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 61 | 375 | 114 | 156 |
| disruptive | 109 | 429 | 202 | 456 |
| missense | 71 | 365 | 166 | 344 |
PlasmoDB Total SNPs: 314
Non-coding: 141 | Synonymous: 111 | Nonsynonymous: 62 | Stop Codon: 0
Protein Length: 3136 | Molecular Weight (kDa): 366.398
UniProt ID(s): Q8I1X5
PDB ID(s): None
Isoelectric Point: 8.94
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR012337 | Ribonuclease H-like superfamily |
| InterPro | IPR012591 | PRO8NT domain |
| InterPro | IPR012592 | PROCN domain |
| InterPro | IPR012984 | PROCT domain |
| InterPro | IPR019580 | Pre-mRNA-processing-splicing factor 8, U6-snRNA-binding |
| InterPro | IPR019581 | Pre-mRNA-processing-splicing factor 8, U5-snRNA-binding |
| InterPro | IPR019582 | RNA recognition motif, spliceosomal PrP8 |
| InterPro | IPR021983 | PRP8 domain IV core |
| InterPro | IPR027652 | Pre-mRNA-processing-splicing factor 8 |
| PFam | PF08082 | PRO8NT domain |
| PFam | PF08083 | PROCN domain |
| PFam | PF08084 | PROCT domain |
| PFam | PF10596 | Pre-mRNA-processing-splicing factor 8, U6-snRNA-binding |
| PFam | PF10597 | Pre-mRNA-processing-splicing factor 8, U5-snRNA-binding |
| PFam | PF10598 | RNA recognition motif, spliceosomal PrP8 |
| PFam | PF12134 | PRP8 domain IV core |
| Superfamily | SSF53098 | Ribonuclease H-like superfamily |
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 12368867 | Sequence of Plasmodium falciparum chromosomes 1, 3-9 and 13. | Hall N, Pain A, ..., Barrell BG | 10.1038/nature01095 |
| 16267556 | A protein interaction network of the malaria parasite Plasmodium falciparum. | LaCount DJ, Vignali M, ..., Hughes RE | 10.1038/nature04104 |
| 23181666 | Organellar proteomics reveals hundreds of novel nuclear proteins in the malariaparasite Plasmodium falciparum. | Oehring SC, Woodcroft BJ, ..., Voss TS | 10.1186/gb-2012-13-11-r108 |
| 30212465 | Integrative proteomics and bioinformatic prediction enable a high-confidenceapicoplast proteome in malaria parasites. | Boucher MJ, Ghosh S, ..., Yeh E | 10.1371/journal.pbio.2005895 |