PF3D7_0405700

Genome location: Pf3D7_04_v3:305,485..313,556(-)

Genome classification: Core

Function and Localization

Product Description: amino acid decarboxylase AAD, putative

SignalP Peptide: N/A

# Transmembrane Domains: 0

EC Numbers: 4.1.1.18 (Lysine decarboxylase)

Curated GO (PlasmoDB):

Type	GO Term	Name
Function	GO:0008923	lysine decarboxylase activity
Process	GO:0006554	lysine catabolic process

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	expressed	N/A	N/A
Ring	possibly expressed	0.10	0.95
Trophozoite	possibly expressed	0.36	0.79
Schizont	expressed	0.22	0.86
Gametocyte	expressed	0.86	0.54

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_0405700

Resistome Missense Mutations: K724N, S1588C

Resistome Compounds with Missense Mutations: MMV665794

Resistome # Samples with Disruptive Mutations: 3 (3 missense, 3 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Mutable in CDS

MIS: 1 | MFS: -1.317 | #Insertions: 10

PlasmoGEM Phenotype: N/A

RMgmDB ABS Phenotype: Not different from wild type (Pb ortholog: PY17X_1004800)

Modification: Disrupted | RMgm-5366

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: Q8I1X1

"Best" AlphaFill ligand hit: PLP (pyridoxal-5'-phosphate, Local RMSD=0.18) with 2X3L (Global RMSD=5.21)

BRENDA EC Inhibitors:

EC #	Name	EC Inhibitors
4.1.1.18	lysine decarboxylase	GTPGDPKClPCMBHgCl2L-ArgCaCl2SO42-MnCl2NaBH4FeCl2ppGpp...

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_110123

No human ortholog(s)

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	40	127	64	156
disruptive	110	339	155	403
missense	67	283	139	348

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	59	241	96	175
disruptive	186	666	266	593
missense	116	547	233	428

PlasmoDB Total SNPs: 335

Non-coding: 128 | Synonymous: 104 | Nonsynonymous: 101 | Stop Codon: 2

Protein Information

Protein Length: 2415 | Molecular Weight (kDa): 280.732

UniProt ID(s): Q8I1X1

PDB ID(s): None

Isoelectric Point: 6.61

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR000310	Orn/Lys/Arg decarboxylase, major domain
InterPro	IPR015424	Pyridoxal phosphate-dependent transferase
InterPro	IPR036633	Orn/Lys/Arg decarboxylase, C-terminal domain superfamily
PFam	PF01276	Orn/Lys/Arg decarboxylase, major domain
Superfamily	SSF53383	Pyridoxal phosphate-dependent transferase
Superfamily	SSF55904	Orn/Lys/Arg decarboxylase, C-terminal domain superfamily