Product Description: V-type proton ATPase subunit B
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: 3.6.3.14 (Transferred entry: 7.1.2.2);7.1.2.2 (H(+)-transporting two-sector ATPase)
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0071944 | cell periphery |
| Component | GO:0020020 | food vacuole |
| Component | GO:0005634 | nucleus |
| Function | GO:0005515 | protein binding |
| Function | GO:0046961 | proton-transporting ATPase activity, rotational mechanism |
| Process | GO:0070070 | proton-transporting V-type ATPase complex assembly |
| Process | GO:0007035 | vacuolar acidification |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | expressed | N/A | N/A |
| Ring | expressed | 0.39 | 0.81 |
| Trophozoite | expressed | 0.85 | 0.54 |
| Schizont | expressed | 0.41 | 0.73 |
| Gametocyte | expressed | 0.78 | 0.58 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_0406100
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Non - Mutable in CDS
MIS: 0.353 | MFS: -2.635 | #Insertions: 0
PlasmoGEM Phenotype: Essential (Pb ortholog: PBANKA_1003800)
RMgmDB ABS Phenotype: N/A
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q6ZMA8
"Best" AlphaFill ligand hit: ADP (adenosine-5'-diphosphate, Local RMSD=0.07) with 5HKK (Global RMSD=2.70)
BRENDA EC Inhibitors:
Orthology to BindingDB Entries:
| UniProt | Protein Name | Species | Homology Source | BindingDB Ligands |
|---|---|---|---|---|
| P15313 | V-type proton ATPase subunit B, kidney isoform | Homo sapiens | HOGENOM, OrthoDB | CHEMBL449696CHEMBL41588CHEMBL39485CHEMBL288419... |
| Q9I8A2 | Vacuolar proton pump subunit B | Gallus gallus | HOGENOM, OrthoDB | bafilomycin A1CHEMBL147307CHEMBL449696CHEMBL148720 |
Ortholog Group (OrthoMCL): OG6_100544
Most Similar Human Ortholog: P15313
TM-align score: 0.96 | RMSD: 1.11
Seq Identity: 0.71 | Length: 478 / 494
All Human Orthologs (OrthoMCL):
| Gene ID | Description |
|---|---|
| ENSG00000116039 | ATPase H+ transporting V1 subunit B1 |
| ENSG00000147416 | ATPase H+ transporting V1 subunit B2 |
Protein Length: 494 | Molecular Weight (kDa): 55.789
PDB ID(s): None
Isoelectric Point: 5.38
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR000194 | ATPase, F1/V1/A1 complex, alpha/beta subunit, nucleotide-binding domain |
| InterPro | IPR004100 | ATPase, F1/V1/A1 complex, alpha/beta subunit, N-terminal domain |
| InterPro | IPR005723 | ATPase, V1 complex, subunit B |
| InterPro | IPR020003 | ATPase, alpha/beta subunit, nucleotide-binding domain, active site |
| InterPro | IPR022879 | V-type ATP synthase regulatory subunit B/beta |
| InterPro | IPR027417 | P-loop containing nucleoside triphosphate hydrolase |
| PFam | PF00006 | ATPase, F1/V1/A1 complex, alpha/beta subunit, nucleotide-binding domain |
| PFam | PF02874 | ATPase, F1/V1/A1 complex, alpha/beta subunit, N-terminal domain |
| Superfamily | SSF52540 | P-loop containing nucleoside triphosphate hydrolase |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 9 | 34 | 27 | 27 |
| disruptive | 0 | 3 | 3 | 20 |
| missense | 0 | 3 | 3 | 17 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 19 | 64 | 27 | 26 |
| disruptive | 1 | 10 | 28 | 76 |
| missense | 1 | 8 | 17 | 54 |
PlasmoDB Total SNPs: 134
Non-coding: 123 | Synonymous: 10 | Nonsynonymous: 1 | Stop Codon: 0
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 7935619 | Cloning and characterization of the vacuolar ATPase B subunit from Plasmodiumfalciparum. | Karcz SR, Herrmann VR, Trottein F, Cowman AF | 10.1016/0166-6851(94)90121-x |
| 12368867 | Sequence of Plasmodium falciparum chromosomes 1, 3-9 and 13. | Hall N, Pain A, ..., Barrell BG | 10.1038/nature01095 |
| 26284889 | Eps15 homology domain containing protein of Plasmodium falciparum (PfEHD)associates with endocytosis and vesicular trafficking towards neutral lipidstorage site. | Thakur V, Asad M, ..., Mohmmed A | 10.1016/j.bbamcr.2015.08.007 |
| 30379851 | Identification of Plasmodium falciparum nuclear proteins by mass spectrometry andproposed protein annotation. | Briquet S, Ourimi A, ..., Vaquero C | 10.1371/journal.pone.0205596 |