Product Description: cytosolic glyoxalase II
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: 3.1.2.6 (Hydroxyacylglutathione hydrolase)
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0005829 | cytosol |
| Function | GO:0004416 | hydroxyacylglutathione hydrolase activity |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | no data | N/A | N/A |
| Ring | no data | 0.24 | 0.88 |
| Trophozoite | no data | 0.44 | 0.73 |
| Schizont | no data | 0.75 | 0.63 |
| Gametocyte | no data | 0.26 | 0.84 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_0406400
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Mutable in CDS
MIS: 0.592 | MFS: -1.357 | #Insertions: 1
PlasmoGEM Phenotype: N/A
RMgmDB ABS Phenotype: Not different from wild type (Pb ortholog: PBANKA_1004000)
Modification: Disrupted | RMgm-4977
More info: PhenoPlasm Link
AlphaFill Uniprot ID: C0H490
"Best" AlphaFill ligand hit: GBP (s-(n-hydroxy-n-bromophenylcarbamoyl)glutathione, Local RMSD=0.17) with 1QH5 (Global RMSD=1.60)
BRENDA EC Inhibitors:
| EC # | Name | EC Inhibitors |
|---|---|---|
| 3.1.2.6 | hydroxyacylglutathione hydrolase | ATPADPAMPGTPGDPCTPUTPKClNEMCMPUMPGSH... |
Orthology to BindingDB Entries:
| UniProt | Protein Name | Species | Homology Source | BindingDB Ligands |
|---|---|---|---|---|
| Q3B7M2 | Hydroxyacylglutathione hydrolase, mitochondrial | HOGENOM, OrthoDB, OrthoMCL BLAST | CHEMBL3629116CHEMBL3629115CHEMBL131578 | |
| O35952 | Hydroxyacylglutathione hydrolase, mitochondrial | Rattus norvegicus | OrthoDB, OrthoMCL BLAST | CHEMBL355031CHEMBL168630 |
| Q16775 | Hydroxyacylglutathione hydrolase, mitochondrial | Homo sapiens | OrthoDB, OrthoMCL BLAST | S-(N-Hydroxy-N-(4-chlorophenyl)carbamoyl)glutathioneS-(N-Hydroxy-N-(4-bromophenyl)carbamoyl)glutathione |
Ortholog Group (OrthoMCL): OG6_101019
Most Similar Human Ortholog: B4DED4
TM-align score: 0.98 | RMSD: 1.00
Seq Identity: 0.37 | Length: 227 / 263
All Human Orthologs (OrthoMCL):
| Gene ID | Description |
|---|---|
| ENSG00000063854 | hydroxyacylglutathione hydrolase |
| ENSG00000103253 | hydroxyacylglutathione hydrolase like |
Protein Length: 263 | Molecular Weight (kDa): 30.528
PDB ID(s): None
Isoelectric Point: 7.74
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR001279 | Metallo-beta-lactamase |
| InterPro | IPR017782 | Hydroxyacylglutathione hydrolase |
| InterPro | IPR032282 | Hydroxyacylglutathione hydrolase, C-terminal domain |
| InterPro | IPR035680 | Hydroxyacylglutathione hydrolase, MBL domain |
| InterPro | IPR036866 | Ribonuclease Z/Hydroxyacylglutathione hydrolase-like |
| PFam | PF00753 | Metallo-beta-lactamase |
| PFam | PF16123 | Hydroxyacylglutathione hydrolase, C-terminal domain |
| Superfamily | SSF56281 | Ribonuclease Z/Hydroxyacylglutathione hydrolase-like |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 4 | 13 | 9 | 11 |
| disruptive | 2 | 8 | 6 | 25 |
| missense | 2 | 8 | 6 | 23 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 7 | 24 | 8 | 20 |
| disruptive | 4 | 33 | 22 | 65 |
| missense | 4 | 25 | 19 | 44 |
PlasmoDB Total SNPs: 154
Non-coding: 146 | Synonymous: 5 | Nonsynonymous: 3 | Stop Codon: 0
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 12368867 | Sequence of Plasmodium falciparum chromosomes 1, 3-9 and 13. | Hall N, Pain A, ..., Barrell BG | 10.1038/nature01095 |
| 15843146 | Characterization of the glyoxalases of the malarial parasite Plasmodiumfalciparum and comparison with their human counterparts. | Akoachere M, Iozef R, ..., Becker K | 10.1515/BC.2005.006 |