PF3D7_0406800

Genome location: Pf3D7_04_v3:351,527..353,137(-)

Genome classification: Core

Function and Localization

Product Description: ribosomal protein L25, putative

SignalP Peptide: N/A

# Transmembrane Domains: 0

EC Numbers: None

Curated GO (PlasmoDB):

Type	GO Term	Name
Component	GO:0022625	cytosolic large ribosomal subunit
Component	GO:0005739	mitochondrion
Function	GO:0008097	5S rRNA binding
Process	GO:0006412	translation

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	not expressed	N/A	N/A
Ring	not expressed	0.02	0.99
Trophozoite	expressed	0.17	0.89
Schizont	possibly expressed	0.04	0.97
Gametocyte	expressed	0.13	0.92

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_0406800

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 5 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Non - Mutable in CDS

MIS: 0.205 | MFS: -2.7 | #Insertions: 0

PlasmoGEM Phenotype: Essential (Pb ortholog: PBANKA_1004400)

Relative Growth Rate: 0.09 ± 0.04
Confidence: 7.70

RMgmDB ABS Phenotype: N/A

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: Q8I1W3

"Best" AlphaFill ligand hit: No AlphaFill hits

No associated EC numbers

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_137035

No human ortholog(s)

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	2	15	13	9
disruptive	4	22	12	33
missense	4	22	12	31

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	6	24	11	20
disruptive	6	39	31	55
missense	6	35	29	48

PlasmoDB Total SNPs: 126

Non-coding: 103 | Synonymous: 15 | Nonsynonymous: 8 | Stop Codon: 0

Protein Information

Protein Length: 286 | Molecular Weight (kDa): 33.86

UniProt ID(s): Q8I1W3

PDB ID(s): None

Isoelectric Point: 7.96

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR011035	Ribosomal protein L25/Gln-tRNA synthetase, anti-codon-binding domain superfamily
InterPro	IPR029751	Ribosomal protein L25
PFam	N/A	N/A
Superfamily	SSF50715	Ribosomal protein L25/Gln-tRNA synthetase, anti-codon-binding domain superfamily

PMID	Title	Authors	DOI/Link
12368867	Sequence of Plasmodium falciparum chromosomes 1, 3-9 and 13.	Hall N, Pain A, ..., Barrell BG	10.1038/nature01095
34494883	A Prioritized and Validated Resource of Mitochondrial Proteins in PlasmodiumIdentifies Unique Biology.	van Esveld SL, Meerstein-Kessel L, ..., Huynen MA	10.1128/mSphere.00614-21