Product Description: peptidyl-tRNA hydrolase 2, putative
SignalP Peptide: N/A
# Transmembrane Domains: 1
EC Numbers: 3.1.1.29 (Aminoacyl-tRNA hydrolase)
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0020011 | apicoplast |
| Component | GO:0005829 | cytosol |
| Component | GO:0005739 | mitochondrion |
| Function | GO:0004045 | aminoacyl-tRNA hydrolase activity |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | expressed | N/A | N/A |
| Ring | expressed | 0.57 | 0.73 |
| Trophozoite | expressed | 0.30 | 0.82 |
| Schizont | expressed | 0.08 | 0.96 |
| Gametocyte | expressed | 0.42 | 0.76 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_0407200
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Non - Mutable in CDS
MIS: 0.297 | MFS: -2.499 | #Insertions: 0
PlasmoGEM Phenotype: Essential (Pb ortholog: PBANKA_1004800)
RMgmDB ABS Phenotype: N/A
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q9U0L4
"Best" AlphaFill ligand hit: No AlphaFill hits
BRENDA EC Inhibitors:
| EC # | Name | EC Inhibitors |
|---|---|---|
| 3.1.1.29 | aminoacyl-tRNA hydrolase | EDTAtRNAtRNALystRNAfMettRNA-lysine5-azacytidine70 S ribosomeUncharged tRNAdeacetylated tRNAdiethyldicarbonatenon-esterified tRNALystRNA-formyl-methionine... |
No evidence of orthology to BindingDB entries
Ortholog Group (OrthoMCL): OG6_100991
Most Similar Human Ortholog: J3KQ48
TM-align score: 0.67 | RMSD: 2.44
Seq Identity: 0.37 | Length: 133 / 189
All Human Orthologs (OrthoMCL):
| Gene ID | Description |
|---|---|
| ENSG00000141378 | peptidyl-tRNA hydrolase 2 |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 2 | 8 | 4 | 14 |
| disruptive | 2 | 20 | 12 | 27 |
| missense | 2 | 20 | 12 | 24 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 3 | 25 | 5 | 17 |
| disruptive | 8 | 54 | 25 | 59 |
| missense | 5 | 50 | 22 | 35 |
PlasmoDB Total SNPs: 20
Non-coding: 17 | Synonymous: 3 | Nonsynonymous: 0 | Stop Codon: 0
Protein Length: 189 | Molecular Weight (kDa): 21.747
UniProt ID(s): Q9U0L4
PDB ID(s): None
Isoelectric Point: 8.82
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR002833 | Peptidyl-tRNA hydrolase, PTH2 |
| InterPro | IPR023476 | Peptidyl-tRNA hydrolase II domain superfamily |
| PFam | PF01981 | Peptidyl-tRNA hydrolase, PTH2 |
| Superfamily | SSF102462 | Peptidyl-tRNA hydrolase II domain superfamily |
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 12368867 | Sequence of Plasmodium falciparum chromosomes 1, 3-9 and 13. | Hall N, Pain A, ..., Barrell BG | 10.1038/nature01095 |
| 30212465 | Integrative proteomics and bioinformatic prediction enable a high-confidenceapicoplast proteome in malaria parasites. | Boucher MJ, Ghosh S, ..., Yeh E | 10.1371/journal.pbio.2005895 |