PF3D7_0407700

Genome location: Pf3D7_04_v3:376,962..383,799(-)

Genome classification: Core

Function and Localization

Product Description: TH1 domain-containing protein, putative

SignalP Peptide: N/A

# Transmembrane Domains: 0

EC Numbers: None

Curated GO (PlasmoDB):

Type	GO Term	Name
Component	GO:0032021	NELF complex
Function	GO:0003723	RNA binding
Process	GO:0034244	negative regulation of transcription elongation from RNA polymerase II promoter

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	not expressed	N/A	N/A
Ring	not expressed	0.12	0.94
Trophozoite	not expressed	0.41	0.77
Schizont	possibly expressed	1.71	0.26
Gametocyte	not expressed	0.90	0.54

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_0407700

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Mutable in CDS

MIS: 1 | MFS: -1.61 | #Insertions: 12

PlasmoGEM Phenotype: Slow (Pb ortholog: PBANKA_1005300)

Relative Growth Rate: 0.91 ± 0.08
Confidence: 6.49

RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PBANKA_1005300)

Modification: Disrupted | RMgm-2723

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: Q9U0K9

"Best" AlphaFill ligand hit: AMP (adenosine monophosphate, Local RMSD=3.65) with 6JWQ (Global RMSD=18.07)

No associated EC numbers

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_134437

No human ortholog(s)

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	12	75	36	94
disruptive	52	194	108	237
missense	31	158	83	198

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	32	149	58	105
disruptive	113	423	183	428
missense	61	305	142	284

PlasmoDB Total SNPs: 340

Non-coding: 167 | Synonymous: 123 | Nonsynonymous: 49 | Stop Codon: 1

Protein Information

Protein Length: 1629 | Molecular Weight (kDa): 197.413

UniProt ID(s): Q9U0K9

PDB ID(s): None

Isoelectric Point: 8.96

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR006942	TH1 protein
PFam	N/A	N/A
Superfamily	N/A	N/A

PMID	Title	Authors	DOI/Link
12368867	Sequence of Plasmodium falciparum chromosomes 1, 3-9 and 13.	Hall N, Pain A, ..., Barrell BG	10.1038/nature01095
16267556	A protein interaction network of the malaria parasite Plasmodium falciparum.	LaCount DJ, Vignali M, ..., Hughes RE	10.1038/nature04104