PF3D7_0407900

Genome location: Pf3D7_04_v3:390,866..394,044(-)

Genome classification: Core

Function and Localization

Product Description: AAA family ATPase, putative

SignalP Peptide: N/A

# Transmembrane Domains: 0

EC Numbers: 3.6.4.3 (Transferred entry: 5.6.1.1)

Curated GO (PlasmoDB):

Type	GO Term	Name
Component	GO:0005634	nucleus
Function	GO:0016887	ATP hydrolysis activity
Function	GO:0008568	microtubule-severing ATPase activity
Process	GO:0031122	cytoplasmic microtubule organization
Process	GO:0010569	regulation of double-strand break repair via homologous recombination

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	expressed	N/A	N/A
Ring	possibly expressed	0.49	0.82
Trophozoite	expressed	0.43	0.74
Schizont	expressed	0.31	0.85
Gametocyte	expressed	0.26	0.84

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_0407900

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Mutable in CDS

MIS: 1 | MFS: -1.68 | #Insertions: 3

PlasmoGEM Phenotype: Dispensable (Pb ortholog: PBANKA_1005500)

Relative Growth Rate: 0.95 ± 0.08
Confidence: 6.37

RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PBANKA_1005500)

Modification: Disrupted | RMgm-2724

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: Q9U0K7

"Best" AlphaFill ligand hit: TAM (tris(hydroxyethyl)aminomethane, Local RMSD=0.16) with 4L15 (Global RMSD=2.61)

BRENDA EC Inhibitors:

EC #	Name	EC Inhibitors
3.6.4.3	microtubule-severing ATPase	No BRENDA inhibitors

Orthology to BindingDB Entries:

UniProt	Protein Name	Homology Source	BindingDB Ligands
Q8I0P1	Spastin	OrthoDB, OrthoMCL	CHEMBL5193085
O75351	Vacuolar protein sorting-associated protein 4B	OrthoDB	CHEMBL4572843CHEMBL4572843CHEMBL4572843
Q9UBP0	Spastin	OrthoDB	CHEMBL5201542CHEMBL5190401

Orthology Information

Ortholog Group (OrthoMCL): OG6_100722

Most Similar Human Ortholog: A0A2R8Y4I8

TM-align score: 0.52 | RMSD: 2.94

Seq Identity: 0.34 | Length: 323 / 667

All Human Orthologs (OrthoMCL):

Gene ID	Description
ENSG00000021574	spastin
ENSG00000132436	fidgetin like 1

Protein Information

Protein Length: 667 | Molecular Weight (kDa): 77.811

UniProt ID(s): Q9U0K7

PDB ID(s): None

Isoelectric Point: 5.12

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR003593	AAA+ ATPase domain
InterPro	IPR003959	ATPase, AAA-type, core
InterPro	IPR003960	ATPase, AAA-type, conserved site
InterPro	IPR027417	P-loop containing nucleoside triphosphate hydrolase
PFam	PF00004	ATPase, AAA-type, core
Superfamily	SSF52540	P-loop containing nucleoside triphosphate hydrolase

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	12	41	24	40
disruptive	13	41	23	90
missense	4	28	23	72

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	25	76	25	33
disruptive	30	114	49	134
missense	6	86	46	92

PlasmoDB Total SNPs: 120

Non-coding: 53 | Synonymous: 49 | Nonsynonymous: 18 | Stop Codon: 0

PMID	Title	Authors	DOI/Link
12368867	Sequence of Plasmodium falciparum chromosomes 1, 3-9 and 13.	Hall N, Pain A, ..., Barrell BG	10.1038/nature01095
16267556	A protein interaction network of the malaria parasite Plasmodium falciparum.	LaCount DJ, Vignali M, ..., Hughes RE	10.1038/nature04104