Product Description: flap endonuclease 1
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: 3.2.2.17 (Deoxyribodipyrimidine endonucleosidase)
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0005634 | nucleus |
| Function | GO:0008409 | 5'-3' exonuclease activity |
| Function | GO:0017108 | 5'-flap endonuclease activity |
| Function | GO:0004520 | endodeoxyribonuclease activity |
| Function | GO:0048256 | flap endonuclease activity |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | expressed | N/A | N/A |
| Ring | not expressed | 0.09 | 0.96 |
| Trophozoite | expressed | 0.83 | 0.56 |
| Schizont | expressed | 0.45 | 0.75 |
| Gametocyte | expressed | 0.82 | 0.58 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_0408500
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Mutable in CDS
MIS: 0.999 | MFS: -0.586 | #Insertions: 2
PlasmoGEM Phenotype: Slow (Pb ortholog: PBANKA_1006100)
RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PBANKA_1006100)
Modification: Disrupted | RMgm-2728
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q9GZ01
"Best" AlphaFill ligand hit: No AlphaFill hits
BRENDA EC Inhibitors:
| EC # | Name | EC Inhibitors |
|---|---|---|
| 3.2.2.17 | deoxyribodipyrimidine endonucleosidase | DNANaBH42-[N-(acetoxyacetyl)amino]fluorene |
Orthology to BindingDB Entries:
| UniProt | Protein Name | Species | Homology Source | BindingDB Ligands |
|---|---|---|---|---|
| P39748 | Flap endonuclease 1 | Homo sapiens | OrthoDB | CHEMBL3615461-(cyclopropylmethyl)-3-hydroxyquinazoline-2,4(1H,3H)-dione (2)3-hydroxy-1-(4-methoxybenzyl)quinazoline-2,4(1H,3H)-dione (4)US9073876, A... |
Ortholog Group (OrthoMCL): OG6_101387
Most Similar Human Ortholog: F5H1Y3
TM-align score: 0.97 | RMSD: 0.87
Seq Identity: 0.56 | Length: 165 / 672
All Human Orthologs (OrthoMCL):
| Gene ID | Description |
|---|---|
| ENSG00000168496 | flap structure-specific endonuclease 1 |
Protein Length: 672 | Molecular Weight (kDa): 76.681
PDB ID(s): None
Isoelectric Point: 7.99
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR002421 | 5'-3' exonuclease |
| InterPro | IPR006084 | XPG/Rad2 endonuclease |
| InterPro | IPR006085 | XPG N-terminal |
| InterPro | IPR006086 | XPG-I domain |
| InterPro | IPR008918 | Helix-hairpin-helix motif, class 2 |
| InterPro | IPR019974 | XPG conserved site |
| InterPro | IPR023426 | Flap endonuclease 1 |
| InterPro | IPR029060 | PIN-like domain superfamily |
| InterPro | IPR036279 | 5'-3' exonuclease, C-terminal domain superfamily |
| PFam | PF00752 | XPG N-terminal |
| PFam | PF00867 | XPG-I domain |
| Superfamily | SSF47807 | 5'-3' exonuclease, C-terminal domain superfamily |
| Superfamily | SSF88723 | PIN-like domain superfamily |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 6 | 31 | 19 | 28 |
| disruptive | 38 | 62 | 28 | 86 |
| missense | 11 | 34 | 24 | 60 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 11 | 60 | 13 | 40 |
| disruptive | 49 | 127 | 44 | 152 |
| missense | 16 | 83 | 34 | 97 |
PlasmoDB Total SNPs: 117
Non-coding: 32 | Synonymous: 37 | Nonsynonymous: 48 | Stop Codon: 0
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 12368867 | Sequence of Plasmodium falciparum chromosomes 1, 3-9 and 13. | Hall N, Pain A, ..., Barrell BG | 10.1038/nature01095 |
| 16267556 | A protein interaction network of the malaria parasite Plasmodium falciparum. | LaCount DJ, Vignali M, ..., Hughes RE | 10.1038/nature04104 |
| 17928073 | Expression and biochemical characterization of the Plasmodium falciparum DNArepair enzyme, flap endonuclease-1 (PfFEN-1). | Casta LJ, Buguliskis JS, Matsumoto Y, Taraschi TF | 10.1016/j.molbiopara.2007.08.008 |
| 23181666 | Organellar proteomics reveals hundreds of novel nuclear proteins in the malariaparasite Plasmodium falciparum. | Oehring SC, Woodcroft BJ, ..., Voss TS | 10.1186/gb-2012-13-11-r108 |
| 30379851 | Identification of Plasmodium falciparum nuclear proteins by mass spectrometry andproposed protein annotation. | Briquet S, Ourimi A, ..., Vaquero C | 10.1371/journal.pone.0205596 |