PF3D7_0408600 (SIAP1)

Genome location: Pf3D7_04_v3:413,403..416,357(+)

Genome classification: Core

Function and Localization

Product Description: sporozoite invasion-associated protein 1

SignalP Peptide: MEGFVALLSFLVVLVFNKTIG

# Transmembrane Domains: 0

EC Numbers: None

Curated GO (PlasmoDB):

Type	GO Term	Name
Component	GO:0045177	apical part of cell
Component	GO:0009986	cell surface

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	expressed	N/A	N/A
Ring	not expressed	0.02	0.99
Trophozoite	not expressed	0.04	0.98
Schizont	not expressed	0.01	0.99
Gametocyte	not expressed	0.10	0.94

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_0408600

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Mutable in CDS

MIS: 1 | MFS: -1.302 | #Insertions: 3

PlasmoGEM Phenotype: Dispensable (Pb ortholog: PBANKA_1006200)

Relative Growth Rate: 0.83 ± 0.18
Confidence: 4.86

RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PBANKA_1006200)

Modification: Disrupted | RMgm-2729

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: Q9U0K0

"Best" AlphaFill ligand hit: MER (meropenem, bound form, Local RMSD=2.50) with 4JF4 (Global RMSD=12.51)

No associated EC numbers

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_130104

No human ortholog(s)

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	4	30	14	56
disruptive	38	27	21	42
missense	38	27	21	38

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	9	50	36	58
disruptive	44	48	29	108
missense	44	48	26	78

PlasmoDB Total SNPs: 38

Non-coding: 0 | Synonymous: 7 | Nonsynonymous: 31 | Stop Codon: 0

Protein Information

Protein Length: 984 | Molecular Weight (kDa): 112.933

UniProt ID(s): Q9U0K0

PDB ID(s): None

Isoelectric Point: 6.72

Protein Domain Annotations:

Source	Family ID	Description
InterPro	N/A	N/A
PFam	N/A	N/A
Superfamily	N/A	N/A

PMID	Title	Authors	DOI/Link
12368867	Sequence of Plasmodium falciparum chromosomes 1, 3-9 and 13.	Hall N, Pain A, ..., Barrell BG	10.1038/nature01095
19181869	Disruption of Plasmodium sporozoite transmission by depletion of sporozoiteinvasion-associated protein 1.	Engelmann S, Silvie O, Matuschewski K	10.1128/EC.00347-08
27128092	Interrogating the Plasmodium Sporozoite Surface: Identification ofSurface-Exposed Proteins and Demonstration of Glycosylation on CSP and TRAP byMass Spectrometry-Based Proteomics.	Swearingen KE, Lindner SE, ..., Sinnis P	10.1371/journal.ppat.1005606
22115782	3D structure and immunogenicity of Plasmodium falciparum sporozoite inducedassociated protein peptides as components of fully-protective anti-malarialvaccine	Alba MP, Almonacid H, ..., Patarroyo ME	10.1016/j.bbrc.2011.11.039