Product Description: perforin-like protein 1
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: None
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0020036 | Maurer's cleft |
| Component | GO:0009986 | cell surface |
| Component | GO:0033644 | host cell membrane |
| Component | GO:0016020 | membrane |
| Component | GO:0020009 | microneme |
| Component | GO:0020005 | symbiont-containing vacuole membrane |
| Function | GO:0005509 | calcium ion binding |
| Function | GO:0022829 | wide pore channel activity |
| Process | GO:0046931 | pore complex assembly |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | expressed | N/A | N/A |
| Ring | possibly expressed | 0.02 | 0.99 |
| Trophozoite | expressed | 0.07 | 0.96 |
| Schizont | expressed | 0.07 | 0.96 |
| Gametocyte | expressed | 0.23 | 0.87 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_0408700
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Mutable in CDS
MIS: 1 | MFS: -1.461 | #Insertions: 1
PlasmoGEM Phenotype: Dispensable (Pb ortholog: PBANKA_1006300)
RMgmDB ABS Phenotype: Not different from wild type (Pb ortholog: PBANKA_1006300)
Modification: Disrupted | RMgm-5268
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q9U0J9
"Best" AlphaFill ligand hit: No AlphaFill hits
No associated EC numbersNo evidence of orthology to BindingDB entries
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 7 | 28 | 26 | 31 |
| disruptive | 57 | 59 | 26 | 71 |
| missense | 51 | 53 | 23 | 59 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 12 | 55 | 26 | 40 |
| disruptive | 73 | 105 | 54 | 106 |
| missense | 62 | 91 | 46 | 81 |
PlasmoDB Total SNPs: 194
Non-coding: 132 | Synonymous: 21 | Nonsynonymous: 41 | Stop Codon: 0
Protein Length: 842 | Molecular Weight (kDa): 94.516
UniProt ID(s): Q9U0J9
PDB ID(s): None
Isoelectric Point: 4.74
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR020864 | Membrane attack complex component/perforin (MACPF) domain |
| PFam | PF01823 | Membrane attack complex component/perforin (MACPF) domain |
| Superfamily | N/A | N/A |
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 12368867 | Sequence of Plasmodium falciparum chromosomes 1, 3-9 and 13. | Hall N, Pain A, ..., Barrell BG | 10.1038/nature01095 |
| 23591903 | Calcium-dependent permeabilization of erythrocytes by a perforin-like proteinduring egress of malaria parasites. | Garg S, Agarwal S, ..., Singh S | 10.1038/ncomms2725 |
| 26292257 | Discovery of Novel Plasmodium falciparum Pre-Erythrocytic Antigens for VaccineDevelopment. | Aguiar JC, Bolton J, ..., Richie TL | 10.1371/journal.pone.0136109 |
| 27128092 | Interrogating the Plasmodium Sporozoite Surface: Identification ofSurface-Exposed Proteins and Demonstration of Glycosylation on CSP and TRAP byMass Spectrometry-Based Proteomics. | Swearingen KE, Lindner SE, ..., Sinnis P | 10.1371/journal.ppat.1005606 |
| 28355563 | Cell Traversal Activity Is Important for Plasmodium falciparum Liver Infection inHumanized Mice. | Yang ASP, O'Neill MT, ..., Boddey JA | 10.1016/j.celrep.2017.03.017 |
| 31631203 | A network-based approach reveals novel invasion and Maurer's clefts-relatedproteins in Plasmodium falciparum. | Das D, Krishnan SR, Roy A, Bulusu G | 10.1039/c9mo00124g |
| 32266171 | Plasmodium Perforin-Like Protein Pores on the Host Cell Membrane Contribute inIts Multistage Growth and Erythrocyte Senescence. | Garg S, Shivappagowdar A, ..., Singh S | 10.3389/fcimb.2020.00121 |
| 38008472 | Structural elucidation of an active polysaccharide from Radix Puerariae lobataeand its protection against acute alcoholic liver disease | Cao W, Wu J, ..., Han J | 10.1016/j.carbpol.2023.121565 |