Product Description: tRNA N6-adenosine threonylcarbamoyltransferase
SignalP Peptide: N/A
# Transmembrane Domains: 1
EC Numbers: 2.3.1.234 (N(6)-L-threonylcarbamoyladenine synthase);2.6.99.4 (Transferred entry: 2.3.1.234)
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0000408 | EKC/KEOPS complex |
| Component | GO:0020011 | apicoplast |
| Function | GO:0003677 | DNA binding |
| Function | GO:0005515 | protein binding |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | expressed | N/A | N/A |
| Ring | possibly expressed | nd | nd |
| Trophozoite | expressed | nd | nd |
| Schizont | expressed | nd | nd |
| Gametocyte | expressed | nd | nd |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_0408900.2
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Non - Mutable in CDS
MIS: 0.138 | MFS: -2.739 | #Insertions: 0
PlasmoGEM Phenotype: Essential (Pb ortholog: PBANKA_1006500)
RMgmDB ABS Phenotype: N/A
More info: PhenoPlasm Link
AlphaFill Uniprot ID: A0A143ZW87
"Best" AlphaFill ligand hit: No AlphaFill hits
BRENDA EC Inhibitors:
| EC # | Name | EC Inhibitors |
|---|---|---|
| 2.3.1.234 | N6-L-threonylcarbamoyladenine synthase | No BRENDA inhibitors |
| 2.6.99.4 | N6-L-threonylcarbamoyladenine synthase | BRENDA SOAP query unsuccessful |
| 2.3.1.234 | N6-L-threonylcarbamoyladenine synthase | No BRENDA inhibitors |
| 2.6.99.4 | N6-L-threonylcarbamoyladenine synthase | BRENDA SOAP query unsuccessful |
No evidence of orthology to BindingDB entries
Ortholog Group (OrthoMCL): OG6_100288
Most Similar Human Ortholog: Q9NPF4
TM-align score: 0.88 | RMSD: 2.43
Seq Identity: 0.19 | Length: 326 / 693
All Human Orthologs (OrthoMCL):
| Gene ID | Description |
|---|---|
| ENSG00000092094 | O-sialoglycoprotein endopeptidase |
| ENSG00000128694 | O-sialoglycoprotein endopeptidase like 1 |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 2 | 37 | 22 | 38 |
| disruptive | 13 | 72 | 48 | 97 |
| missense | 13 | 70 | 46 | 91 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 6 | 67 | 24 | 55 |
| disruptive | 30 | 157 | 77 | 160 |
| missense | 24 | 133 | 67 | 116 |
PlasmoDB Total SNPs: 170
Non-coding: 147 | Synonymous: 11 | Nonsynonymous: 12 | Stop Codon: 0
Protein Length: 693 | Molecular Weight (kDa): 80.85
UniProt ID(s): Q9U0J7
PDB ID(s): None
Isoelectric Point: 9.62
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR000905 | Gcp-like domain |
| InterPro | IPR034680 | tRNA N6-adenosine threonylcarbamoyltransferase Kae1, archaea and eukaryote |
| InterPro | IPR043129 | ATPase, nucleotide binding domain |
| PFam | PF00814 | Gcp-like domain |
| Superfamily | SSF53067 | ATPase, nucleotide binding domain |
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 12368867 | Sequence of Plasmodium falciparum chromosomes 1, 3-9 and 13. | Hall N, Pain A, ..., Barrell BG | 10.1038/nature01095 |
| 16267556 | A protein interaction network of the malaria parasite Plasmodium falciparum. | LaCount DJ, Vignali M, ..., Hughes RE | 10.1038/nature04104 |
| 34033682 | Boosting endoplasmic reticulum folding capacity reduces unfolded protein responseactivation and intracellular accumulation of human kidney anion exchanger 1 inSaccharomyces cerevisiae | Li X, Cordat E, Schmitt MJ, Becker B | 10.1002/yea.3652 |
| 31996424 | Saccharomyces cerevisiae: First Steps to a Suitable Model System To Study theFunction and Intracellular Transport of Human Kidney Anion Exchanger 1 | Sarder HAM, Li X, ..., Becker B | 10.1128/mSphere.00802-19 |
| 31417508 | KAE1 Allelic Variants Affect TORC1 Activation and Fermentation Kinetics inSaccharomyces cerevisiae | Kessi-Perez EI, Salinas F, ..., Martinez C | 10.3389/fmicb.2019.01686 |
| 36416748 | Kae1 of Saccharomyces cerevisiae KEOPS complex possesses ADP/GDP nucleotidaseactivity | Li QX, Liu JC, He MH, Zhou JQ | 10.1042/BCJ20220290 |