PF3D7_0409300

Genome location: Pf3D7_04_v3:440,536..445,079(-)

Genome classification: Core

Function and Localization

Product Description: methyltransferase, putative

SignalP Peptide: N/A

# Transmembrane Domains: 0

EC Numbers: 2.1.1.201 (2-methoxy-6-polyprenyl-1,4-benzoquinol methylase)

Curated GO (PlasmoDB): None

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	not expressed	N/A	N/A
Ring	expressed	0.63	0.70
Trophozoite	expressed	0.69	0.63
Schizont	expressed	0.13	0.92
Gametocyte	expressed	0.53	0.71

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_0409300

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Mutable in CDS

MIS: 0.654 | MFS: -3.042 | #Insertions: 1

PlasmoGEM Phenotype: Dispensable (Pb ortholog: PBANKA_1006900)

Relative Growth Rate: 1.03 ± 0.13
Confidence: 5.51

RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PBANKA_1006900)

Modification: Disrupted | RMgm-2735

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: Q9U0J3

"Best" AlphaFill ligand hit: SAH (s-adenosyl-l-homocysteine, Local RMSD=5.21) with 2PXX (Global RMSD=7.83)

BRENDA EC Inhibitors:

EC #	Name	EC Inhibitors
2.1.1.201	2-methoxy-6-polyprenyl-1,4-benzoquinol methylase	No BRENDA inhibitors

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_100611

Most Similar Human Ortholog: P0DPD7

TM-align score: 0.75 | RMSD: 3.26

Seq Identity: 0.19 | Length: 224 / 1010

All Human Orthologs (OrthoMCL):

Gene ID	Description
ENSG00000010165	eEF1A lysine and N-terminal methyltransferase
ENSG00000284753	EEF1A lysine methyltransferase 4

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	15	43	28	56
disruptive	54	150	67	186
missense	24	113	50	145

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	18	95	32	64
disruptive	93	276	107	226
missense	44	196	90	161

PlasmoDB Total SNPs: 188

Non-coding: 98 | Synonymous: 49 | Nonsynonymous: 41 | Stop Codon: 0

Protein Information

Protein Length: 1010 | Molecular Weight (kDa): 120.659

UniProt ID(s): Q9U0J3

PDB ID(s): None

Isoelectric Point: 7.1

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR029063	S-adenosyl-L-methionine-dependent methyltransferase
PFam	N/A	N/A
Superfamily	SSF53335	S-adenosyl-L-methionine-dependent methyltransferase